《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (12): 971-972.

• 专题 • 上一篇    下一篇

β淀粉样肽引起的大鼠脑神经髓鞘结构与MBP变化及金思维的影响

徐意; 田金洲; 盛树力; 时晶; 姬志鹃; 尹军祥; 赵志炜   

  1. 1.北京中医药大学东直门医院老年医学研究所 北京市 100700;2.首都医科大学宣武医院脑老化重点实验室 北京市 100053
  • 收稿日期:2005-09-09 出版日期:2005-12-25 发布日期:2005-12-25

Changes of brain myelin sheath structure and myelin basic protein content induced by amyloid β peptide (Aβ) and effect of GETO on these changes

XU Yi, TIAN Jin-zhou, SHENG Shu-li, et al   

  1. Geriatrics Institute, Dongzhimen Hospital, Beijing University of Traditional Chinese Medicine, Beijing 100070, China
  • Received:2005-09-09 Published:2005-12-25 Online:2005-12-25

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摘要： 目的观察β淀粉样肽(Aβ)引起的大鼠脑有髓神经髓鞘结构与髓鞘碱性蛋白(MBP)改变,及金思维对其的影响。方法采用Aβ1-42海马注射复制痴呆模型。电镜观察有髓神经髓鞘的结构;免疫组化方法显示MBP在海马区的分布和含量。结果电镜显示模型组大鼠海马区神经髓鞘结构松解紊乱和均质化,可见缺失;金思维组结构完整,板层连续,与正常组相似。MBP免疫组化显示模型组大鼠海马区髓鞘着色明显低于正常组,分支短少而不连续;金思维组髓鞘着色较浅,但数目较多而长。模型组大鼠的MBP阳性突起数、平均面积和平均光密度与正常组和金思维组均有非常显著性差异(P<0.01)。结论Aβ海马注射可造成大鼠脑有髓神经髓鞘损伤,MBP缺失,髓鞘结构破坏;金思维对上述变化有一定改善作用。

关键词: β淀粉样肽, 髓鞘, 髓鞘碱性蛋白

Abstract: ObjectiveTo observe the injured changes of brain myelin sheath structure and myelin basic protein (MBP) content induced by amyloid β peptide (Aβ) and effect of GETO on these changes.MethodsThe experimental rat model of Alzheimer's disease was established with Aβ1-42 injection into hippocampus. 4 weeks later, the myelin sheath structure of the CA1 area of the rat hippocampus was taken and observed by electromicroscope, and distribution and content of MBP were examined with immunohistochemical method.ResultsThe electromicroscope showed that the structure of myelin sheath became relaxing, disorder, homogenization and default of hippocampus CA1 in the model rats. In GETO treated group, the structure of myelin sheath was integrity and continuum. Immunohistochemical test showed that the staining and numbers of myelin sheath of model rats was thinner than that of normal rats and GETO treated rats. The numbers, mean area and mean density of positive staining axon in hippocampus CA1 of MBP in the model rats were significantly different from those in the normal group and GETO group (P<0.01).ConclusionAβ1-42 injection into hippocampus in rats can impair myelin sheath to make MBP release and GETO can ameliorate these changes.

Key words: amyloid β peptide, myelin sheath, myelin basic protein (MBP)