《中国康复理论与实践》

《中国康复理论与实践》 ›› 2005, Vol. 11 ›› Issue (12): 973-974.

• 专题 • 上一篇    下一篇

当归注射液对大鼠脑缺血再灌注损伤后VEGF表达的影响

郑婵娟; 廖维靖; 杨万同; 蒙兰青   

  1. 武汉大学中南医院康复医学科 湖北武汉市 430071
  • 收稿日期:2005-08-03 出版日期:2005-12-25 发布日期:2005-12-25

Effects of Angelica sinensis on the expression of vascular endothelial growth factor after the brain ischemia-reperfusion injury in rats

ZHENG Chan-juan, LIAO Wei-jing, YANG Wan-tong, et al   

  1. The Department of Rehabilitation Medicine, Zhongnan Hospital of Wuhan University,430071 Wuhan, Hubei, China
  • Received:2005-08-03 Published:2005-12-25 Online:2005-12-25

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摘要： 目的观察大鼠局灶性脑缺血再灌注损伤后血管内皮生长因子(VEGF)在不同时间点的表达及当归注射液对其表达的影响。方法线栓法制作脑缺血再灌注损伤模型,雄性Wistar大鼠随机分为正常对照组、缺血损伤组和当归治疗组,采用免疫组织化学和RT-PCR方法观察VEGF表达变化。结果免疫组织化学显示缺血损伤组和当归治疗组大鼠VEGF蛋白表达均在24h达到高峰后减弱;RT-PCR显示缺血损伤组VEGF mRNA在3h开始表达增强,6h达到高峰,后很快降低,至第7天恢复到基线水平;当归治疗组VEGF mRNA在3d达到高峰后缓慢降低,至第7天仍有大量表达,且各时间点VEGF的表达比缺血损伤组明显增加。结论当归可促进局灶性脑缺血再灌注损伤后VEGF的表达。

关键词: 脑缺血/再灌注损伤, 血管内皮生长因子, 当归注射液

Abstract: ObjectiveTo investigate the effects of Angelica sinensis on the expression of vascular endothelial growth factor (VEGF) Flt-1, Flk-1 mRNA after the brain ischemia-reperfusion injury in rats.MethodsWistar rats were randomly divided into the group A, group B and normal control group. The group A underwent middle cerebral artery occlusion (MCAO) for 2h by suture, group B underwent MCAO for 2h meanwhile received treatment with Angelica sinensis (5 g/kg). Immunohistochemistry and quantitative reverse transcription and polymerase chain reaction (RT-PCR) technique were used to examine the gene expression of VEGF.ResultsThe result of immunohistry revealed that VEGF in the group A and group B reached its peak at 24 h after reperfusion then declined gradually. The result of RT-PCR manifested that the gene expression of VEGF in the group A increased from 3 h after reperfusion and reached its peak at 6 h; in the group B reached its peak on the 3rd day. The expression of VEGF in the group B was significantly increased than group A at the same time point.ConclusionAngelica sinensis can enhance the expression of Flt-1, Flk-1 after transient interruption of cerebral blood flow in rats.

Key words: brain ischemia-reperfusion injury, vascular endothelial growth factor (VEGF), Angelica sinensis injection