《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (05): 383-384.

• 专题 • 上一篇    下一篇

两种微囊化大鼠胰岛异种移植于糖尿病小鼠的疗效比较

徐琦; 窦科峰; 宋振顺; 张福琴   

  1. 第四军医大学西京医院肝胆外科 陕西西安市 710032
  • 收稿日期:2006-03-25 出版日期:2006-05-25 发布日期:2006-05-25

Comparison of the Efficacy for Diabetic Mice Xenotransplanted with Two Types of Microencapsulated Rat Islets

XU Qi, DOU Ke-feng, SONG Zhen-shun, et al   

  1. Department of Surgery of Liver and Gallbladder, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, Shaanxi, China
  • Received:2006-03-25 Published:2006-05-25 Online:2006-05-25

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摘要： 目的将大鼠胰岛分别用两种不同的免疫隔离材料微囊化移植到糖尿病小鼠肾包膜下,对它们的疗效进行比较。方法尾静脉注射3%链脲佐菌素建立小鼠糖尿病模型。成模小鼠随机分为4组:海藻酸钡微囊化胰岛移植组、琼脂糖聚苯乙烯磺酸微囊化胰岛移植组、未微囊化胰岛移植组和空白对照组,每只小鼠肾包膜下植入300个相应大鼠胰岛。结果4组糖尿病小鼠移植前血糖水平无显著差异,移植后1周分别为:海藻酸钡微囊化胰岛移植组(7.26±1.56)mmol/L、琼脂糖聚苯乙烯磺酸微囊化胰岛移植组(7.14±1.04)mmol/L、未微囊化胰岛移植组(7.42±1.52)mmol/L、空白对照组(22.54±1.24)mmol/L,前2组与后2组相比有显著性差异,生存期也明显长于后2组;海藻酸钡微囊化移植组维持正常血糖时间及生存期(分别为76d和92d)明显长于琼脂糖微囊化移植组(分别为41d和56d)。结论用海藻酸钡和琼脂糖聚苯乙烯磺酸制备的微囊化大鼠胰岛均可存活于糖尿病小鼠体内并发挥生物功能,海藻酸钡微囊优于琼脂糖聚苯乙烯磺酸。

关键词: 糖尿病, 微囊化, 海藻酸钡, 琼脂糖聚苯乙烯磺酸, 异种移植, 胰岛, 大鼠, 小鼠

Abstract: ObjectiveTo observe and compare the efficacy of two types of microencapsulated rat islets xenotransplanted into diabetic mice. MethodsThe mice diabetic model made with injecting 3% Streptozotosin through tail vein. Four groups were assigned: control group, naked islet transplantation group, alginate-BaCl2 microencapsulated islet transplantation group, agarose-PSSa microencapsulated islet transplantation group.300 islets were transplanted under the renal envelope of each diabetic mice respectively. ResultsThere were no significant difference in mean level of the blood glucose before transplantation among four groups. One week after transplantation, the respective mean level of the blood glucose in four groups were (7.26±1.56) mmol/L in alginate-BaCl2 microencapsulated islet transplantation group, (7.14±1.04) mmol/L in agarose-PSSa microencapsulated islet transplantation group, (7.42±1.52) mmol/L in naked islet transplantation group and (22.54±1.24) mmol/L in control. There were significant difference between the two encapsulated islet groups and the other two groups. The survived period of the two encapsulated islet transplantation groups were longer than that of the other two groups. The survived period of the alginate-BaCl2 microencapsulated islet transplantation group was longer than that of the agarose-PSSa microencapsulated islet transplantation group (92 d vs 56 d),the same as the time of keeping nomal blood glucose level (76 d vs 41 d). ConclusionMicroencapsulated rat islets with this two materials can survive in diabetic mice with their biological activity, and the alginate-BaCl2 microcapsules are better than the agarose-PSSa microcapsules.

Key words: diabetes mellitus, microencapsulation, alginate-BaCl2, agarose-PSSa, xenotransplantation, islet, rat, mouse