《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (08): 692-693.

• 基础研究 • 上一篇    下一篇

MMPs抑制剂对退变腰椎间盘中MMP-3表达的影响

陈德胜1; 金群华1; 李燕2; 杨文君2; 昌晓红3   

  1. 1.宁夏医学院附属医院骨科,宁夏银川市 750004;2.宁夏医学院基础学院生物化学与分子生物学教研室,宁夏银川市 750004;3.北京大学人民医院妇科肿瘤实验室,北京市 100044
  • 收稿日期:2006-02-23 出版日期:2006-08-01 发布日期:2006-08-01

Effect of Metalloproteinases Inhibitor on Expression of MMP-3 in Degenerated Lumbar Intervertebral Disc

CHEN De-sheng, JIN Qun-hua, LI Yan, et al   

  1. The Department of Orthopedics, the Affiliated Hospital of Ningxia Medical College, Yinchuan 750004, Ningxia, China
  • Received:2006-02-23 Published:2006-08-01 Online:2006-08-01

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摘要： 目的探讨基质金属蛋白酶(MMPs)抑制剂对退变腰椎间盘中MMP3的作用。方法采用大鼠建立腰椎间盘退变动物模型;将32只大鼠随机分为实验组和对照组各16只。造模4周后,实验组动物每日皮下注射四环素25mg,对照组注射生理盐水,2周后取椎间盘组织,采用免疫组织化和Western-bloting法检测MMP3的表达。结果实验组大鼠椎间盘组织MMP3表达低于对照组(P<0.05)。结论MMPs抑制剂能降低退变腰椎间盘中MMP3的表达。

关键词: 腰椎间盘, 退变, 基质金属蛋白酶, 抑制剂

Abstract: ObjectiveTo explore the effect of metalloproteinases (MMPs) inhibitor on expression of MMP-3 in degenerated lumbar intervertebral disc.MethodsThe animal model of degenerated lumbar intervertebral disc was established with rats. The 32 rats were randomly divided into the experimental group and control group with 16 animals in each group. From the fourth week after operation, the animals of the experimental group were injected with tetracycline 25 mg per day, those of the control group with saline. Two weeks late, the tissue of degenerated lumbar intervertebral disc was taken and the expression of MMP-3 was tested by immumohistochemistry and Western-bloting.ResultsThe expression of MMP-3 in the experimental group decreased and significantly different from the control group ( P<0.05).ConclusionMMPs inhibitor can decrease expression of MMP-3 in degenerated lumbar intervertebral disc.

Key words: lumbar intervertebral disc, degeneration, metalloproteinases, inhibitor