《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (08): 694-695.

• 基础研究 • 上一篇    下一篇

用基因芯片表达谱筛选恶性黑色素瘤差异基因的研究

张琳西1; 易成刚1; 张旭东1; 杨力1; 杨继庆2; 郭树忠1   

  1. 1.第四军医大学西京医院整形外科研究所,陕西西安市 710032;2.第四军医大学生物医学工程系,陕西西安市 710032
  • 收稿日期:2006-04-28 出版日期:2006-08-01 发布日期:2006-08-01

Study of Screening Differential Genes of Malignant Melanoma Using Gene Chip Expression Profiles

ZHANG Lin-xi, YI Cheng-gang,ZHANG Xu-dong, et al   

  1. TheInstitute of Plastic Surgery, XijingHospital, FourthMilitary Medical University, Xi'an 710032, Shaanxi, China
  • Received:2006-04-28 Published:2006-08-01 Online:2006-08-01

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摘要： 目的用基因芯片表达谱筛选出恶性黑色素瘤的差异基因。方法用Agilent Human1 Aoligo DNA芯片对恶性黑色素瘤和色素痣组织中基因表达情况进行检测,提取总RNA,进行荧光标记探针、杂交、洗涤后分析。结果恶性黑色素瘤和正常色素痣组织共有1596个差异表达基因,其中上调基因733个,下调基因863个。结论基因芯片技术可筛选出恶性黑色素瘤相关基因。

关键词: 基因芯片, 恶性黑色素瘤, 色素痣, 差异基因

Abstract: ObjectiveTo identify differential genes of malignant melanoma using gene chip expression profiles.MethodsAgilent Human 1A OligoDNA was employed to find out difference in gene expression between malignant melanoma and nevus. The total RNA was isolated from two type tissues, labled the fluorescent to the probe, hybridized, washed and analyzed.ResultsAmong the 21073 target genes, 1596 genes were differentially expressed in malignant melanoma, including 733 genes up-regulated, and 863 down-regulated.ConclusionThe gene chip technique can screen genes that may be specifically expressed in malignant melanoma.

Key words: gene chip technique, malignant melanoma, nevus, differential gene