《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (08): 696-698.

• 基础研究 • 上一篇    下一篇

艾炷灸贴预处理对AA大鼠足肿胀率及应激激素的影响

郑玲1a; 李晓泓1a; 张露芬1a; 倪健1b; 李辉2; 周登芳1a; 翟景慧1a; 何玉伟1a   

  1. 1.北京中医药大学a.针灸学院;b.药学院,北京市 100029;2.中日友好医院,北京市 100013
  • 收稿日期:2006-03-03 出版日期:2006-08-01 发布日期:2006-08-01

Effect of Dressing and Moxibustion-pretreatment on Local Feet Swelling and Stress Hormone in Hypothalami of Rats with Adjuvant Arthritis at Early and Secondary Stages

ZHENG Ling, LI Xiao-hong, ZHANG Lu-fen, et al   

  1. The School of Acupuncture, Beijing University of Traditional Chinese Medicine, Beijing 100029, China
  • Received:2006-03-03 Published:2006-08-01 Online:2006-08-01

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被引次数

28

摘要： 目的观察艾炷灸贴预处理对随后佐剂性关节炎(AA)大鼠早期和继发期足肿胀率及下丘脑应激激素的影响。方法将40只Wistar大鼠随机分为正常组、模型早期组和继发组、预灸贴早期组和继发组共5组,于造模前用中药膜剂贴敷大鼠大椎穴后加艾灸,观察其对随后AA大鼠下丘脑促肾上腺皮质激素释放激素(CRH)、β内啡肽(βEP)、神经肽Y(NPY)及足肿胀率的影响。结果造模后,模型早期组和继发组大鼠的右足肿胀率明显升高(P<0.01),预灸贴各组较同期模型组降低(P<0.05～0.01);下丘脑CRH水平造模后升高(P<0.05～0.01),但预灸贴早期组较同期模型组有下调趋势,预灸贴继发组下降(P<0.05);βEP水平造模后早期组明显升高(P<0.01),继发组变化不大;与同期模型组相比,预灸贴早期组βEP明显下降,而继发组βEP却明显升高(均P<0.01);下丘脑NPY水平造模后升高(P<0.05～0.01),预灸贴继发组NPY较同期模型组下降(P<0.05)。结论艾炷灸贴预处理可减轻随后AA大鼠足肿胀程度,机理可能与其调节下丘脑CRH、βEP及NPY水平有关。

关键词: 艾炷灸贴预处理, 佐剂性关节炎, 应激, 促肾上腺皮质激素释放激素, β-内啡肽, 神经肽Y

Abstract: ObjectiveTo observe the effect of dressing and moxibustion-pretreatment on the local joint swelling and stress hormone in hypothalami of rats with adjuvant arthritis (AA) at early and secondary stages.MethodsForty Wistar rats were randomly divided into 5 groups: normal group, early and secondary model groups, early and secondary pre-dressing and moxibustion (PDM) groups. The dressing with Chinese herb and moxibustion was stuck on Dazhui point (GV14) before the AA model established. The effects of dressing and moxibustion-pretreatment on the feet swelling and corticotropin-releasing-hormone (CRH), beta-endorphin (β-EP) and neuropeptide-Y (NPY) in hypothalami were observed.ResultsThe right feet swelling rate at early and secondary stages obviously increased after modeling ( P<0.01), and it became lower in early and secondary PDM groups than in model groups at the same phases ( P<0.05～0.01). The level of hypothalamic CRH was higher after modeling ( P<0.05～0.01), compared with early model group, it had a tendency to going down in early PDM group, moreover, in secondary PDM group the level was similar with the normal group. The level of hypothalamic β-EP increased significantly in early model group ( P<0.01), and lightly changed in secondary model group, it decreased in early PDM group but increased in secondary PDM group, compared with model groups at the same stages ( P<0.05). The level of hypothalamic NPY increased significantly after modeling, and it declined in the secondary PDM group ( P<0.05).ConclusionDressing and moxibustion-pretreatment can relief feet swelling of AA rats, which may be related with its regulative effect on the level of hypothalamic CRH, β-EP and NPY.

Key words: dressing and moxibustion-pretreatment, adjuvant arthritis, stress, corticotropin-releasing-hormone, beta-endorphin, neuropeptide-Y