《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (08): 701-703.

• 基础研究 • 上一篇    下一篇

巴戟天水提物对人精子运动功能氧化损伤的保护作用

颜志中; 杨欣; 张永华; 丁彩飞; 杜静   

  1. 浙江省中西医结合医院男科,浙江杭州市 310003
  • 收稿日期:2006-04-05 出版日期:2006-08-01 发布日期:2006-08-01

Protective Effect of Extract from Morindae Officinalis on the Oxidative Injury of Human Sperm Motility

YAN Zhi-zhong, YANG Xin, ZHANG Yong-hua, et al   

  1. Zhejiang Province Cooperation of Chinese and Western Medicine Hospital, Hangzhou 310003,Zhejiang, China
  • Received:2006-04-05 Published:2006-08-01 Online:2006-08-01

PDF (PC)

摘要： 目的观察巴戟天水提物对人精子运动功能氧化损伤的保护作用。方法采用体外培养精子模型,应用次黄嘌呤黄嘌呤氧化酶(HXXO)体系产生活性氧,在有氧环境下,将活性氧和不同剂量的巴戟天水提物与精子悬液共同孵育后,检测精子运动参数,应用精子毛细管穿透试验评估精子运动功能,并与已知的抗氧化剂VitC对照。结果在活性氧作用下,精子的功能受损,运动功能和穿透能力明显下降,与正常精子相比有非常高度显著性差异(P<0.001);巴戟天水提物大、中、小剂量组在相同条件下均可明显改善精子的运动功能和穿透能力,与VitC相比较,0.25和0.5g/ml巴戟天水提物的作用更明显(P<0.05,P<0.01),尤其对前向运动率和活力指数的改善最明显。结论活性氧可导致精子运动功能和穿透能力的损伤,适宜剂量的巴戟天水提物对活性氧所致人精子过氧化损伤具有明显干预作用,对精子运动功能具有保护作用。

关键词: 巴戟天, 精子, 氧化损伤

Abstract: ObjectiveTo observe the protective effect of extract from Morindae officinalis on the oxidative injury of human sperm motility.MethodsThe sperm specimens were cultivated in vitro, the reactive oxygen species (ROS) was made from hypoxanthine-xanzine xanzine (HX-XO), and sperm specimens were co-incubated with ROS and different dosages of extract and oxygen. The motor parameters were evaluated, the motility were analyzed by the test of capillary tube penetrating, and compared with the control group.ResultsThe sperm function were injured, the motility and penetrating ability decreased evidently under the action of ROS, and with a significant difference compared with the normal group ( P<0.001). The sperm motility and penetrating ability could be improved by all of the small, medium and massive extract compared with vitamin C, especially 0.25 and 0.5 g/ml Morindae officinalis had more evident action in the rate of progressive motility and vitality exponent.ConclusionThe ROS can injury the sperm motility and penetrating ability, the appropriate content of extract from Morindae officinalis can significantly intervene peroxidation in sperm by the ROS, and protect the sperm motility.

Key words: Morindae officinalis, sperm, oxidative injury