《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (11): 957-959.

• 基础研究 • 上一篇    下一篇

半定量和定性方法评价截瘫大鼠后肢运动功能恢复的比较

张缨1,2; 纪树荣1,2; 范晓华1,2; 周红俊1,2; 刘根林1,2   

  1. 1.首都医科大学康复医学院,北京市 100068;2.北京博爱医院,北京市 100068
  • 收稿日期:2006-07-26 出版日期:2006-11-01 发布日期:2006-11-01

Semiquantitative and Qualitative Assessment of Functional Recovery after Spinal Cord Injury in Adult Rats

ZHANG Ying, JI Shu-rong, FAN Xiao-hua, et al   

  1. Faculty of Rehabilitaion of Capital Medical University, Beijing Charity Hospital, Beijing 100068, China
  • Received:2006-07-26 Published:2006-11-01 Online:2006-11-01

PDF (PC)

被引次数

15

摘要： 目的了解胸髓完全横断大鼠后肢运动功能恢复的特点;探讨评价截瘫大鼠后肢运动恢复的最佳方法。方法26只成年W istar大鼠随机分为假手术组、脊髓横断损伤组和减重平板步行训练组,分别于术前和术后1 d、7 d、15 d、30 d和45 d,采用BBB开放空间运动评分和ACOS(average comb ined scores)神经行为学评定观察大鼠后肢运动功能的恢复情况,并比较其异同点。结果术后1 d大鼠后肢呈弛缓性瘫痪,BBB和ACOS评分均为0分;30 d和45 d时与损伤组比较,训练组大鼠后肢运动功能恢复明显,BBB和ACOS评分均存在显著性差异。术后15 d与30 d、45 d,30 d与45 d相比,损伤组和训练组内ACOS评分均存在显著性差异。损伤组与训练组BBB和ACOS评分的Pearson相关系数分别为0.991与0.987。结论胸髓完全横断大鼠仍存在部分后肢运动功能自发恢复的征象,减重平板步行训练能促进截瘫大鼠后肢运动功能的恢复。半定量和定性方法的评价结果均能较好地体现损伤后运动功能的恢复情况,两者相关性高。其中半定量方法能更敏感地反映不同时间点不同组别间运动恢复的细微变化。

关键词: 脊髓损伤, 运动功能, 康复, 评定, 大鼠

Abstract: ObjectiveTo investigate the characteristic of hindlimb movement recovery in adult rats after spinal cord injury and how to assess it.Methods 26 adult female rats were randomized in sham,completely midthoracic spinal cord transected(at T8 level) or body weight supported treadmill training(BWSTT) groups.Their motor function were assessed with Basso,Beattie,Bresnahan locomotor rating scale for open field(BBB scale) and average combined scores(ACOS).ResultsNone of the rats exhibited hindlimb movements after operation.30 d and 45 d after operation,BBB and ACOS score of rats in BWSTT group showed better than that in SCI group.There were significant differences in ACOS score among 15 d,30 d and 45 d after operation in both group.ConclusionPartial spontaneous hindlimb functional recovery can be found in chronic adult spinal cord injured rats and BWSTT would facilitate the recovery.Both semiquantitative and qualitative assessment were strongly correlated with locomotor recovery,but the semiquantitative assessment shows more sensitive.

Key words: spinal cord injury, locomotor function, rehabilitation, assessment, rats