《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (12): 1056-1057.

• 基础研究 • 上一篇    下一篇

小鼠再生肝浸出液体外诱导骨髓间充质干细胞向肝细胞样分化的研究

于俊兰1,2; 李荣1; 王文杰3; 李力1; 刘巨超1; 王金晶1; 徐迎新1   

  1. 1.解放军总医院普通外科研究所,北京市 100853;2.解放军291医院,内蒙古包头市 014040;3.清华大学材料系生物材料实验室,北京市 100084
  • 收稿日期:2006-07-17 出版日期:2006-12-01 发布日期:2006-12-01

Bone Marrow Mesenchymal Stem Cells Differentiated into Hepatocyte-like Cells Induced by Rebirth Liver Tissue Lixivium of Mouse in Vitro

YU Jun-lan, LI Rong, WANG Wen-jie, et al   

  1. The Institute of General Surgery, the General Hospital of PLA, Beijing 100853, China
  • Received:2006-07-17 Published:2006-12-01 Online:2006-12-01

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被引次数

5

摘要： 目的应用小鼠再生肝浸出液体外诱导骨髓间充质干细胞向肝细胞样分化。方法利用2/3肝部分切除术后36h小鼠再生肝组织浸出液定向诱导小鼠骨髓间充质干细胞,在倒置显微镜下观察细胞形态,应用免疫荧光法鉴定细胞性质。结果骨髓间充质干细胞经小鼠再生肝浸出液诱导7d后,呈肝细胞样圆形,1～2周后免疫荧光染色可见细胞表达肝细胞标记物CK8、CK18和清蛋白。结论小鼠再生肝浸出液可诱导骨髓间充质干细胞向肝细胞方向分化。

关键词: 骨髓间充质干细胞, 肝样细胞, 分化, 组织工程

Abstract: Objective To induce bone marrow mesenchymal stem cells (BMSCs) differentiated into hepatocyte-like cells with rebirth liver tissue lixivium of mouse in vitro.Methods Mouse BMSCs were isolated and directionally induced with rebirth liver tissue lixivium of mouse 36 h after partial hepatectomy (PH). The morphology of cells was observed under an invert microscope, and the characteristics of differentiated cells was identified by immunofluorescence test.Results 7 days after induced by liver lixivium, the spindle shaped BMSCs became round and resembled hepatocyte-like cells. 1～2 weeks later, the differentiated cells expressed albumin, CK8 and CK18, which were known as characteristic markers of the hepatocyte.Conclusion BMSCs of mouse can be differentiated into hepatocyte-like cells under induction of liver tissue lixivium of mouse.

Key words: bone marrow mesenchymal stem cells (BMSCs), hepatocyte-like cells, differentiation, tissue engineering