《中国康复理论与实践》

《中国康复理论与实践》 ›› 2006, Vol. 12 ›› Issue (12): 1060-1062.

• 基础研究 • 上一篇    下一篇

差异表达基因Hepsin在前列腺癌中的表达及其意义

梁文立1,2,3; 周利群1,2; 辛殿琪1,2; 那彦群1,2   

  1. 1.北京大学第一医院泌尿外科,北京市 100034;2.北京大学泌尿外科研究所,北京市 100034;3.北京博爱医院泌尿外科,北京市 100068
  • 收稿日期:2006-04-12 出版日期:2006-12-01 发布日期:2006-12-01

Expression and Significance of Differential Expression Gene Hepsin in the Prostate Cancer

LIANG Wen-li, ZHOU Li-qun, XIN Dian-qi, et al   

  1. The Department of Urology, the First Hospital of Peking University, Beijing 100034, China
  • Received:2006-04-12 Published:2006-12-01 Online:2006-12-01

PDF (PC)

摘要： 目的探讨cDNA芯片筛选出的前列腺癌(PC)差异表达基因Hepsin在PC中的表达及其意义。方法应用RT-PCR检测Hepsin在正常前列腺组织、PC组织及PC细胞系中的表达,并进行半定量分析,同时采用免疫印迹法检测Hepsin的表达,采用免疫组织化学染色法检测40例PC标本、15例前列腺增生(BPH)标本和6例正常前列腺标本的Hepsin表达水平。结果在mRNA水平,Hepsin在PC中的表达水平高于正常前列腺组织(P=0.026),但在PC细胞系中的表达并不增高。在蛋白表达水平,正常前列腺组织未表达Hepsin,而PC及PC细胞系均表达Hepsin。PC和BPH标本免疫组化检测均为阳性,但两者间有非常高度显著性差异(P=0.000),而正常前列腺标本为阴性。结论Hepsin在PC中的表达增高,有可能作为早期诊断的标志以及基因治疗的靶基因。

关键词: 前列腺癌, 基因表达, Hepsin基因

Abstract: Objective To evaluate the expression and significance of differential expression gene Hepsin in the prostate cancer (PC) screened by the cDNA microarray technique.Methods The techniques of semiquantitative RT-PCR and Western blotting were used to detect the mRNA and protein expression of Hepsin. Specimens of 40 cases of PC, 15 benign prostatic hyperplasia (BPH) and 6 normal prostate tissues were examined by the immunohistochemical stain.Results Hepsin was more expressed in PC tissue than normal prostate tissue (P=0.026) and was confirmed by Western blot analysis. Immunohistochemical test confirmed this and demonstrated that Hepsin did not expressed in normal prostate but expressed in PC and BPH and there was a significant difference in Hepsin expression level between PC and BPH tissues ( P=0.000).Conclusion Hepsin high expressed in PC may be a new molecular marker in early diagnosis of PC and a new target for gene therapy of PC.

Key words: prostate cancer, gene expression, Hepsin gene