《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (02): 183-185.

• 临床研究 • 上一篇    下一篇

神经梅毒患者外周血淋巴细胞、树突状细胞亚群研究

张海萍1a; 连石1a; 朱威1a; 孙雪静1b   

  1. 1.首都医科大学宣武医院a.皮肤性病科;b.血液科,北京市 100053
  • 收稿日期:2006-12-13 出版日期:2007-02-01 发布日期:2007-02-01

Study of the Subsets of the Lymphocyte and Dendritic Cells in Peripheral Blood in Patients with Neurosyphilis

ZHANG Hai-ping,LIAN Shi, ZHU Wei, et al   

  1. The Department of Dermatology, Xuanwu Hospital of the Capital Medical University, Beijing 100053, China
  • Received:2006-12-13 Published:2007-02-01 Online:2007-02-01

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摘要： 目的分析神经梅毒患者外周血中淋巴细胞、树突状细胞的亚群特点,探讨其与神经梅毒发病之间的关系。方法分别取健康人(10例,对照组)和神经梅毒患者(8例,试验组)外周血,用荧光标记法经流式细胞仪检测CD4+T、CD8+T、Th1、Th2细胞及DC1、DC2细胞的比例。结果神经梅毒患者外周血中的CD4+T细胞比例高于正常对照组(P<0.05);CD8+T细胞比例低于正常对照组(P<0.05)。在CD4+T细胞中,神经梅毒患者外周血中的Th1、Th2细胞分别占(14.12±5.12)%、(1.52±0.88)%,Th1/Th2比例为(12.05±5.62);正常对照组Th1、Th2细胞分别占(26.10±4.98)%、(0.99±0.35)%,Th1/Th2比例为(31.62±16.62)。神经梅毒患者外周血中的Th1细胞及Th1/Th2的比例低于正常对照组(P<0.05)。神经梅毒患者外周血中的DC1、DC2细胞分别占(0.44±0.17)%和(0.25±0.19)%,正常对照组分别占(0.34±0.10)%和(0.26±0.13)%,两组间差异无显著性意义(P>0.05)。神经梅毒患者的DC1与Th1、DC2与Th2存在相关关系(P<0.05),DC1、DC2与Th1/Th2无相关关系(P>0.05)。结论神经梅毒患者细胞免疫功能受抑,其外周血中DC亚群的比例与Th1/Th2失衡无关。

关键词: 神经梅毒, 树突状细胞, 淋巴细胞, 流式细胞仪

Abstract: ObjectiveTo analysis the subsets of the lymphocyte and dendritic cells (DC) in peripheral blood in patients with neurosyphilis and investigate the relationship between these cells and onset of neurosyphilis.MethodsThe subsets of CD4+T cells as well as DCs and the counts of CD4+T and CD8+T were analyzed by flow cytometry with immunofluorescent staining in 8 patients with neurosyphilis and 10 healthy controls.ResultsIn peripheral blood of neurosyphilis patients the proportion of CD4+T cell was significantly higher than that in the normal controls ( P<0.01); the proportion of CD8+T cell was significantly lower than that in the normal controls ( P<0.05). The proportion of Th1, Th2 and the ratio of Th1/Th2 in neurosyphilis patients was (14.12±5.12)%, (1.52±0.88)% and (12.05±5.62), respectively. In normal controls, it was (26.10±4.98)%, (0.99±0.35)% and (31.62±16.62) respectively. The expression of Th1 and the ratio of Th1/Th2 in neurosyphilis patients was significantly lower than the normal controls (P<0.05). The proportion of DC1 and DC2 in neurosyphilis patients was not significantly different from that in normal controls ( P>0.05). There was a significant correlation between DC1 and Th1, DC2 and Th2 in the neurosyphilis patients ( P<0.051), but no correlation between the proportion of DCs and the ratio of Th1/Th2 ( P>0.05).ConclusionThe cellular immune function of neurosyphilis patients may be inhibited, and there is no relationship between the proportion of DCs subset and unbalance of Th1/Th2.

Key words: neurosyphilis, dendritic cell, lymphocyte, flow cytometry