《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (03): 248-251.

• 基础研究基础研究 • 上一篇    下一篇

低频振动对骨髓基质细胞成骨能力影响及机制的研究

叶超群1,2; 纪树荣3; 杨键3; 潘立冬3; 王子羲4   

  1. 1.首都体育学院保健康复教研室,北京市 100088;2.北京军区总医院骨科,北京市 100700;3.中国康复研究中心,北京市 100068;4.清华大学精密仪器系,北京市 100084
  • 收稿日期:2005-10-31 出版日期:2007-03-01 发布日期:2007-03-01

Influence of low frequency vibration on osteogenesis of bone marrow stromal cells and its mechanism

YE Chao-qun, JI Shu-rong,YANG Jian,et al   

  1. Teaching & Research Section of Health Care & Recovery, Capital Institute of Physical Education, Beijing 100088, China
  • Received:2005-10-31 Published:2007-03-01 Online:2007-03-01

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被引次数

13

摘要： 目的了解低频振动对骨髓基质细胞(BMSCs)成骨能力及其OPG基因、RANKL基因表达的影响。方法分离、培养10周龄雌性SD大鼠BMSCs,随机分为静止培养对照组和振动组,振动组于培养的第11天开始接受振动干预7d;振动结束后对两组BMSCs的增殖、碱性磷酸酶(ALP)、钙化结节及OPGmRNA、RANKLmRNA进行检测。结果振动组BMSCs增殖能力较对照组明显提高(P<0.05),但ALP活性、钙化结节无明显改变。振动组BMSCsOPG基因表达明显上调(P<0.05),RANKL基因表达无明显变化。结论低频振动可促进BMSCs的增殖,可能与其促进OPG基因表达上调有关;但未发现能使BMSCs成骨能力出现明显改变。

关键词: 低频振动, 骨髓基质细胞(BMSCs), 成骨能力, OPG, RANKL

Abstract: Objective To explore the effect of low frequency vibration (LFV) on osteogenesis of bone marrow stromal cells (BMSCs) and OPG mRNA,RANKL mRNA expression.MethodsBMSCs from 10-week-old female SD rats were cultured and randomly divided into 2 groups:control group and vibration group.The BMSCs of vibration group was given LVF with 20 Hz,0.25 g,2 times/d,10 min/time for 7 days.The LFV was given from the eleventh day after BMSCs cultured.The osteogenesis and OPG mRNA,RANKL mRNA of all BMSCs in two group were tested at the end of LFV.ResultsCompared to the control groups,the BMSCs growth and proliferation improved in vibration group,but the alkaline phosphatase (ALP)activity and the number of mineral nodule did not changed significantly.The OPG mRNA elevated in vibration group,while the RANKL mRNA showed no change.ConclusionThe LFV may increase the growth and proliferation of BMSCs,which is attributed to the elevated OPG gene expression.The osteogenesis improved no significance,which may be associated with the too short vibration time.

Key words: low frequency vibration, bone marrow stromal cells, osteogenesis, OPG, RANKL