《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (05): 401-403.

• 专题　缺血性脑卒中 •    下一篇

脑缺血预适应大鼠脑内细胞外信号调节激酶磷酸化水平和蛋白表达量的改变

赵兰峰; 郭社卫; 安仰原; 刘明刚   

  1. 首都医科大学基础医学院神经生物学系疼痛生物医学研究所,北京市 100069
  • 收稿日期:2007-03-23 出版日期:2007-05-01 发布日期:2007-05-01

Phosphorylation and Expression of Extracellular Signal-regulated Kinase in Cerebral Ischemic Preconditioning Rats

ZHAO Lan-feng,GUO She-wei, AN Yang-yuan, et al   

  1. Institute for Biomedical Science of Pain, Departmentof Neurobiology, Capital Medical U- niversity School of Basic Medical Science, Beijing 100069, China
  • Received:2007-03-23 Published:2007-05-01 Online:2007-05-01

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摘要： 目的初步探讨细胞外信号调节激酶(ERK1/2)在大鼠脑缺血预适应中(IPC)的作用。方法将大鼠随机分为正常对照(control)、假手术(SI)、缺血预适应或缺血耐受(IT)、单纯蜜蜂毒(BV)、外周伤害耐受(PNT)等5组。应用SDS-PAGE、Western blot等实验方法,借助GelDoc和VersaDoc凝胶成像系统,半定量检测脑躯体感觉皮层、海马组织内ERK的磷酸化水平和蛋白表达量的变化。结果经脑IPC大鼠躯体感觉皮层内ERK1的磷酸化水平(激活程度)增高(P<0.05);大脑躯体感觉皮层内ERK2及海马组织内ERK1/ERK2的磷酸化水平无明显变化。大脑躯体感觉皮层和海马组织内ERK1/ERK2经脑IPC后蛋白表达量均无明显变化。结论大鼠大脑躯体感觉皮层ERK1磷酸化水平的增高可能参与了脑缺血预适应的形成。

关键词: 脑缺血预适应, ERK1/2, 磷酸化, 蛋白表达

Abstract: Objective To explore initially the role of extracellular signal-regulated kinase (ERK1/2) in cerebral ischemic preconditioning. Methods Healthy adult SD rats were randomly divided into 5 groups: normal control group; sham group; ischemic preconditioning or ischemia tolerance group; bee venom group; peripheral noxious tolerance group. SDS-PAGE, Western blot and Gel Doc imagine systems were applied to determine the ERK1/2 phosphorylation and protein expression in somatosensory cortex and hippocampus of rats. Results The phosphorylation level of ERK1 in somatosensory cortex increased significantly (P<0.05) after ischemic preconditioning, while no significant changes in ERK2 and that of ERK1/2 in hippocampus. No significant changes in ERK1/2 protein expression were found both in somatosensory cortex and hippocampus after ischemic preconditioning. Conclusion The increased ERK1 phosphorylation level in somatosensory cortex may be involved in cerebral ischemic preconditioning.

Key words: cerebral ischemic preconditioning, extracellular signal-regulated kinase (ERK1/2), phosphorylation, protein expression