《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (05): 425-427.

• 基础研究 • 上一篇    下一篇

黏附分子在大鼠骨骼肌缺血再灌注损伤中的作用

王新涛1; 吕松岑2; 韩竹2; 孙宇一3; 闫景龙1   

  1. 1.哈尔滨医科大学附属第一医院骨科,黑龙江哈尔滨市 150001;2.哈尔滨医科大学附属第二医院骨科,黑龙江哈尔滨市 150086;2.哈尔滨市公安医院,黑龙江哈尔滨市 150001
  • 收稿日期:2007-02-15 出版日期:2007-05-01 发布日期:2007-05-01

Effect of Adhesion Molecules on Skeletal Muscle Ischemia Reperfusion Injury

WANG Xin-tao, Lü Song-cen, HAN Zhu, et al   

  1. Department of Orthopaedic Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang,China
  • Received:2007-02-15 Published:2007-05-01 Online:2007-05-01

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摘要： 目的探讨大鼠骨骼肌缺血再灌注(I/R)时黏附分子的动态变化及其在骨骼肌I/R损伤中的作用。方法42只Wistar大鼠随机分为正常对照组(n=6)、缺血组(n=6)、缺血再灌注组(n=30)。分别于缺血期,再灌注1、2、4、8、12h测定白细胞上黏附分子β2整合素(CD11b/CD18)和骨骼肌血管中细胞间黏附分子-1(ICAM-1)的表达情况,测定血浆中的丙二醛、骨骼肌组织中的髓过氧化物酶,及各组的组织学改变。结果随着骨骼肌再灌注的时间的延长,黏附分子的表达逐渐增多,再灌注8～12h表达最多,骨骼肌组织损伤也随再灌注时间的延长而逐渐加重,时程上与黏附分子表达同步。结论黏附分子的表达与骨骼肌I/R损伤密切相关。

关键词: 缺血再灌注损伤, 骨骼肌, β2整合素, 细胞间黏附分子-1

Abstract: Objective To investigate the changes of adhesion molecules and their effects on skeletal muscle ischemia/reperfusion injury. Methods 42 Wistar rats were divided into 3 groups: normal control group (Group Ⅰ, n=6), ischemia group (Group Ⅱ, n=6),ischemia/reperfusion injury group (Group Ⅲ, n=30). The level of malondialdehyde (MDA) in the plasma, myeloperoxidase (MPO) in the skeletal muscle, CD11b/CD18 on the leukocytes, intercelluar adhesion molecule-1 (ICAM-1) in the skeletal muscle and the histological changes were studied 1 h, 2 h, 4 h, 8 h, 12 h reperfusion after ischemia for 4 h. Results In group Ⅲ, the expression of CD11b/CD18, ICAM-1 and the injury of skeletal muscle increased with the lapse of reperfusion time. They reached the peak at 8～12 hours' reperfusion. The injury of skeletal muscle developed with the expression of adhesion molecules. Conclusion The expression of CD11b/CD18 and ICAM-1 are significantly associated with the skeletal muscle ischemia-reperfusion injury.

Key words: ischemia-reperfusion injury, skeletal muscle, CD11b/CD18, intercelluar adhesion molecule-1 (ICAM-1)