《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (05): 430-431.

• 基础研究 • 上一篇    下一篇

转染mtrⅡ基因后霍奇金病细胞增殖活性改变

王星1; 林雨冬1; 王明元1; 师建国2; 吴德柱1; 王煜1; 杜京丽1   

  1. 1.南京军区福州总医院95临床部,福建莆田市 351100;2.第四军医大学基础部病理教研室,陕西西安市 710032
  • 收稿日期:2006-12-21 出版日期:2007-05-01 发布日期:2007-05-01

Proliferative Activities of Hodgkin's Disease Cells Transfected with mtrⅡ Gene

WANG Xing, LIN Yu-dong, WANG Ming-yuan, et al   

  1. The95th Clinical Department of PLA Fuzhou General Hospital of Nanjing Military Area, Putian 351100, Fujian, China
  • Received:2006-12-21 Published:2007-05-01 Online:2007-05-01

PDF (PC)

被引次数

7

摘要： 目的探讨转染mtrⅡ基因后的霍奇金病细胞增殖活性的改变及其意义。方法将mtrⅡ转染人霍奇金病细胞系,采用免疫组化法检测转染前后ki67基因、增殖细胞核抗原(PCNA)表达情况。结果未转染mtrⅡ基因的ki67阳性率为21.3%,转染mtrⅡ基因后ki67阳性率为36.8%,两者间有显著性差异(P<0.05);未转染mtrⅡ基因的PCNA阳性率为46.1%,转染mtrⅡ基因后PCNA的阳性率为78.6%,两者间有显著性差异(P<0.05)。结论转染mtrⅡ基因后的霍奇金病细胞增殖活性明显增加,提示mtrⅡ基因可能参与了霍奇金病恶性转化的过程。

关键词: mtrⅡ基因, 霍奇金病, ki67基因, 增殖细胞核抗原(PCNA)

Abstract: Objective To investigate the proliferative activities and significance of Hodgkin's disease cells which were transfected with mtr Ⅱ gene. Methods The ki67 and proliferating cell nuclear antigen (PCNA) expression in Hodgkin's disease cells transfected with mtr Ⅱ gene were determined with immunohistochemistry. Results The ki67 and PCNA expression in cells transfected with mtr Ⅱ gene was more than that of cells without mtrⅡ gene transfected (P<0.05). Conclusion The proliferative activities of Hodgkin's disease cells transfected with mtr Ⅱ gene increased obviously, which may be related to the progress of Hodgkin's disease.

Key words: mtr Ⅱ gene, Hodgkin's disease, ki67 gene, proliferating cell nuclear antigen (PCNA)