《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (05): 434-437.

• 基础研究 • 上一篇    下一篇

自体骨髓单核细胞移植对急性心肌梗死的作用(英文)

姜雪松; 朱智涛; 刘文华   

  1. 哈尔滨医科大学附属第二医院重症治疗病房,黑龙江省哈尔滨市 150086
  • 收稿日期:2006-12-07 出版日期:2007-05-01 发布日期:2007-05-01

Effect of Bone-Marrow Mononuclear Cells Transplantation on Acute Myocardial Infarction

JIANG Xue-song1, ZHU Zhi-tao2, LIU Wen-hua1   

  1. 1.ICU, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang, China; 2. Department of Cardiovascular Surgery, The First Affiliated Hospital of Harbin　Medical University, Harbin 150001, Heilongjiang, China.
  • Received:2006-12-07 Published:2007-05-01 Online:2007-05-01

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摘要： 目的探讨未经诱导的自体骨髓单核细胞可否在梗死心肌环境中存活并分化为心肌细胞及血管内皮细胞。方法40只日本大耳雄兔随机分为两组:移植组及对照组,每组各20只。采用结扎冠状动脉左前降支的方法建立急性心梗模型,以心电图证实模型成功,由超声心动图评价心功能。模型建立后7天,将BrdU标记的自体骨髓单核细胞注射到移植组动物心肌梗死区及周边区,而对照组动物相同部位仅注射等量生理盐水。移植后6周,收集动物心脏进行组织学及免疫组化分析。结果抗BrdU免疫组化发现移植组动物心肌梗死区及周边区内均存在染色阳性的移植细胞,且周边区内的移植细胞呈心肌细胞及血管内皮细胞的形态特点,同时这些细胞抗心肌特异性肌动蛋白抗体染色阳性,证实其肌源性分化。另外,移植组动物梗死周边区血管密度显著高于对照组(P<0.05),但两组动物在心肌梗死区内的血管密度没有统计学差异(P>0.05)。移植后6周,两组动物心功能均有改善,移植组明显优于对照组(P<0.05)。结论自体骨髓单核细胞移植于梗死心肌后,可在梗死区及周边区存活,并在周边区分化为血管内皮细胞及具有心肌细胞形态特点的细胞、增加梗死周边区的血管密度,改善心功能。

关键词: 骨髓单核细胞, 自体, 细胞移植, 心肌梗死

Abstract: Objective To investigate whether uninduced autologous bone-marrow mononuclear cell (ABM-MNC) could survive and differentiate into myocardial cells and endothelial cells in the infarcted heart. Methods 40 male big-ear Japanese rabbits were divided into two groups randomly: the transplanted group (n=20) and the control group (n=20). The model of acute myocardial infarction was made by left anterior descending artery ligation, which was confirmed by ECG. The cardiac function was evaluated by the echocardiography. 7 days later, BrdU labeled ABM-MNCs were injected into infarcted and marginal area myocardium in the transplanted group, while the control rabbits were injected with saline. 6 weeks later, the hearts were harvested for histology and immunohistochemistry evaluation. Results In the transplanted group, viable cells labeled with BrdU could be identified in the infarcted area, and myocytes and endothelial cells labeled with BrdU can also be found in the border area, these cells demonstrate myogenic differentiation with the expression of α-Actin by immunostaining. Moreover, the vessel density of the transplanted group in the borders of the infarction was higher than the control group (P<0.05), but there was no difference in the infarcted areas between two groups (P>0.05). At the 6 weeks after experiment, the cardiac function was improved in both groups, but the transplanted group improved more than that in the control group (P<0.05). Conclusion Autologous bone-marrow mononuclear cells injected into the infarcted myocardium could survive in both the infarcted and the border areas, differentiated into endothelial cells and other cells which have obtained the characters of myocytes, and increase the vessel density in border area, improved the cardiac function.

Key words: bone-marrow mononuclear cells, autologous, cell transplantation, myocardial infarction