《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (06): 501-503.

• 专题　脊柱脊髓损伤康复 •    下一篇

大鼠脊髓损伤后bFGF、EGF海绵对内源性神经干细胞的诱导及修复作用

刘海1; 洪涛2; 王忠诚1; 安沂华1; 崔勇1   

  1. 1.首都医科大学附属北京天坛医院,北京市 100050;2.安顺市人民医院神经外科,贵州安顺市 561400
  • 收稿日期:2007-05-08 出版日期:2007-06-01 发布日期:2007-06-01

Induction and Repair of bFGF and EGF Sponge to Endogenous Neural Stem Cells after Half Cut-off of Spine Cord in Rats

LIU Hai,HONG Tao, WANG Zhong-cheng, et al   

  1. Beijing TianTan Hospital, the Capital Medical University, Beijing 100050, China
  • Received:2007-05-08 Published:2007-06-01 Online:2007-06-01

PDF (PC)

被引次数

23

摘要： 目的探讨碱性成纤维细胞生长因子(bFGF)、表皮生长因子(EGF)海绵植入损伤脊髓对脊髓损伤后内源性干细胞的诱导修复作用。方法采用脊髓半横断制作脊髓损伤模型,将46只大鼠分为对照组(n=6,不损伤脊髓)、模型组(n=20)和实验组(n=20,造模后应用bFGF、EGF海绵治疗),对3组动物进行行为学评分、电生理、电镜及免疫组化检测。结果实验组动物经bF-GF、EGF海绵治疗后,运动功能高于模型组,内源性神经干细胞数量多于模型组(P<0.05)。结论bFGF、EGF海绵干预可促进脊髓损伤动物内源性神经干细胞增殖、分化,改善受损的脊髓功能。

关键词: 脊髓损伤, 碱性成纤维细胞生长因子, 表皮生长因子海绵, 内源性神经干细胞, 大鼠

Abstract: Objective To investigate induction and repair of basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) sponge to endogenous neural stem cells after acute spinal cord half cut-off in rats.MethodsThe differences of proliferation and differentiation of endogenous neural stem cells were compared between injured group and intervention treatment group.ResultsThere are remarkable differences between the injured group and intervention treatment group.ConclusionbFGF and EGF sponge can enhance the proliferation and differentiation of the neural stem cells in the test animal.

Key words: spinal cord injury, basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF) sponge, endogenous neural stem cell, rat