《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (06): 528-530.

• 基础研究 • 上一篇    下一篇

HIV-慢病毒载体有效转导人肺动脉平滑肌细胞的实验研究

李寿霖1,2; 服部俊夫3a; 池田淳3b; 白土邦男3b   

  1. 1.首都医科大学康复医学院,北京市 100068; 2.中国康复研究中心北京博爱医院内科,北京市 100068;3.日本东北大学大学院医学系研究科a.感染病态学;b.循环病态学,日本仙台市980-8574
  • 收稿日期:2007-04-05 出版日期:2007-06-01 发布日期:2007-06-01

Experimental Study of Efficient Transduction for Human Pulmonary Artery Smooth Muscle Cells with HIV-based Lentivirus Vectors

LI Shou-lin, HATTORIToshio, IKEDA Jun, et al   

  1. CapitalMedical University School of Rehabilitation Medicine, ChinaRehabilitation Research Center, Beijing 100068, China
  • Received:2007-04-05 Published:2007-06-01 Online:2007-06-01

PDF (PC)

被引次数

5

摘要： 目的使用不同的HIV-载体感染人肺动脉平滑肌细胞,并使用HIV慢病毒载体将外源基因转入人肺动脉平滑肌细胞。方法应用ADA/Luc、HXB2/Luc、VSV-G/Luc和VSV-G/GFP感染人肺动脉平滑肌细胞U87.CD4.CCR5和U87.CD4.CX-CR4。结果ADA/Luc和HXB2/Luc均能感染具有相应复合受体的U87.CD4,但不能感染人肺动脉平滑肌细胞。VSV-G/Luc和VSV-G/GFP能有效转导至人肺动脉平滑肌细胞中,于感染第3天,表达VSV-G/GFP的人肺动脉平滑肌细胞的阳性率为18%。结论应用HIV慢病毒载体能够向人肺动脉平滑肌细胞中导入外源基因。

关键词: 平滑肌细胞, 肺动脉高压, 载体, 人免疫缺陷病毒

Abstract: Objective To infect human pulmonary artery smooth muscle cells with different HIV-based vectors, and introduce foreign genes into the cells with HIV-based lentivirus vectors.MethodsThe human pulmonary artery smooth muscle cells, U87.CD4.CCR5 and U87.CD4.CXCR4, were infected with ADA/Luc, HXB2/Luc, VSV-G/Luc and VSV-G/GFP.ResultsU87.CD4 with corresponding coreceptors could be infected by ADA/Luc and HXB2/Luc, however, the human pulmonary artery smooth muscle cells could not be infected by ADA/Luc and HXB2/Luc, even pseudotypes. VSV-G/Luc and VSV-G/GFP could be transfered into the human pulmonary artery smooth muscle cells. After 3 days post-infection, the relative proportion of positive cells of human pulmonary artery smooth muscle cells expressing VSV-G/GFP was 18%.ConclusionHIV-based lentivirus vectors can introduce foreign genes into the human pulmonary artery smooth muscle cells.

Key words: smooth muscle cell, pulmonary hypertension, vector, human immunodeficiency virus (HIV)