《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (07): 626-629.

• 基础研究 • 上一篇    下一篇

G-CSF动员后外周血干细胞移植的成心肌和血管作用的实验研究(英文)

刘文华; 陈永胜; 姜雪松; 于凯江; 李海波   

  1. 哈尔滨医科大学附属第二医院ICU,黑龙江哈尔滨市 150086
  • 收稿日期:2006-11-28 出版日期:2007-07-01 发布日期:2007-07-01

Granulocyte-Colony Stimulating Factor Mobilizing Autologous Peripheral Blood Stem Cells Transplantation to Form Cardiac Myoid Cells and Angiogenesis

LIU Wen-hua1,CHEN Yong-sheng2,JIANG Xue-song1,YU Kai-jiang1,LI Hai-bo1   

  1. ICU, The Second Clinical College, Harbin Medical University, Harbin 150086, Heilongjiang, China; 2. Department of Urology, Tumour Hospital, Harbin Medical University, Harbin 150040, Heilongjiang, China
  • Received:2006-11-28 Published:2007-07-01 Online:2007-07-01

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摘要： 目的研究粒细胞集落刺激因子(G-CSF)动员后自体外周血干细胞(PBSCs)移植的成心肌和血管的作用。方法60只日本大耳白兔随机分成移植组、动员剂组和对照组,每组20只,均采用结扎冠状动脉前降支的方法建立心肌梗死(MI)模型,移植组连续给予G-CSF7d,在模型建立后1周,将分离外周血所得的干细胞用BrdU标记,制成细胞悬液经心外膜注射到心梗区及周边,对照组只给予等量的生理盐水注射。组织学分析检测植入细胞的存活及分化情况,并测定毛细血管密度。结果移植组在梗死区可发现BrdU阳性心肌样细胞,且表达Actin阳性。HE染色显示移植组梗死区细胞排列有序,可见大量的新生毛细血管,而对照组梗死区结构紊乱,毛细血管密度较移植组明显降低(P<0.01)。Masson染色对照组胶原纤维明显多于移植组和动员剂组。结论G-CSF动员后植入自体外周血单个核细胞可在梗死区内及周边区存活并分化成心肌样细胞,同时明显促进了毛细血管的生成。

关键词: 外周血单个核细胞, 心肌样细胞, 新生血管, 心肌梗死

Abstract: Objective To study autologous peripheral blood stem cells (PBSCs) transplantation for cardiac myoid cells formation and angionesis after recombinant human granulocyte-colony stimulating factor (rhG-CSF) mobilizing. Methods The 60 white Japanese big-ear rabbits were divided into 3 groups, i.e. transplantation (T) mobiliztor (M) and control (C) groups, each group with 20 rabbits. Myocardial infarction (MI) model was developed by ligating the anterior descending coronary artery. G-CSFs were given continually for 7 d in T and M groups since 1 h after MI model development. Cell suspension which derived from the peripheral blood and labeled with BrdU which prepared 1 week ago were injected into infarction regions and borders, while in C groups only the same doses saline was injected. The survival and differentiation of the implanted cells were detected with histological analyses and capillary densities. Results BrdU positive cells which were taken on immature cardiac myoid cells were observed at infarcted areas in T group, which were detected as the Actin positively. HE stains showed that the structures of infarction regions were deranged in C group, but in T and M groups cell arrangements were arranged regularly. Meanwhile, there were a large amount of neogenesis capillaries. The capillaries densities were respectively (58.2±11.5) and (52.3±6.0) per high-power field in T and M groups, while in C group was (21.6±4.9) (P<0.01). In C group blue collagen fibers were much more than T and M group under Masson stains (P<0.01). In T and M groups the cardiac functions were much better than in the C group at the end of 4 weeks, especially ejection fraction were respectively (65.34±2.54)% and (63.40±2.84)% in M and T groups. In C group it was only (50.51±6.47)% (P<0.01).Conclusion After G-CSF mobilizing the implanted PBSCs may survive and differentiate into cardiac myoid cells in infarcted areas and vicinities, at the same time promote neogenisis and improve cardiac function. It is significant that cell transplantation will treat the cardiac infarction in future.

Key words: peripheral blood stem cell, cardiac myoid cells, angiogenesis, myocardial infarction