《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (09): 839-841.

• 基础研究 • 上一篇    下一篇

莫诺苷对SH-SY5Y神经细胞生长的影响及对过氧化氢诱导损伤的保护

黄文婷1; 王文2; 艾厚喜2; 安宜1; 李林2; 王玉兰2   

  1. 1.北京理工大学生命科学与技术学院生物技术系,北京市 100081;2.首都医科大学宣武医院药物研究室,教育部神经变性病学重点实验室,北京市 100053
  • 收稿日期:2007-04-28 出版日期:2007-09-01 发布日期:2007-09-01

Influence of Morroniside on the Growth and Protection Against H_2O_2-Induced Cytotoxicity in SH-SY5Y Nerve Cells

HUANG Wen-ting, WANG Wen, AI Hou-xi, et al   

  1. School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081,China
  • Received:2007-04-28 Published:2007-09-01 Online:2007-09-01

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摘要： 目的研究从中药山茱萸分离提取的有效成分莫诺苷对人神经母细胞瘤株SY5Y生长的影响及对H2O2诱导SH-SY5Y细胞损伤的神经保护作用。方法对培养的SH-SY5Y细胞加入不同浓度的莫诺苷,以MTT代谢率为指标确定无毒范围以及莫诺苷促进神经细胞生长的最佳有效浓度;对培养的SH-SY5Y细胞加入最佳有效浓度的莫诺苷,观察MTT代谢率、细胞轴突长度及胞体面积、细胞计数的改变;H2O2500μmol/L诱导SH-SY5Y细胞损伤,加入不同浓度的莫诺苷及维生素E,以WesternBlotting检测神经生长因子(NGF)的表达。结果莫诺苷在10～100μmol/L浓度范围内均可增加SH-SY5Y细胞的存活率;培养的SH-SY5Y细胞分别加入10μmol/L莫诺苷,与正常对照组相比,莫诺苷组组MTT代谢率升高(P<0.05),细胞轴突长度显著增长(P<0.001),胞体面积明显增加(P<0.01),细胞计数增加(P<0.05);H2O2损伤后,莫诺苷组NGF的表达明显高于对照组(P<0.01)。结论莫诺苷对SH-SY5Y细胞的生长有促进作用,并对H2O2诱导的损伤有神经保护作用。

关键词: 莫诺苷, 神经细胞, 神经营养, 神经保护

Abstract: Objective To investigate the effect of Morroniside which was isolated from Cornus officinalis on the growth and the protection against H2O2-induced injury in SH-SY5Y neuroblastoma cells.Methods SH-SY5Y cells were incubated with Morroniside in different concentration.MTT metabolic rate was measured as the cell survival rate in order to determine the best effective concentration to promote the survival of neuron.MTT metabolic rate,axonal length,area of cell body and cell counting were used as the indicators to analyze the effects of Morroniside on the growth of SH-SY5Y on the best effective concentration.After SH-SY5Y cells were injured with 500 μmol/L H2O2,they were administrated with Morroniside in different concentration and vitamin E.The expression of neural growth factor(NGF)were determined with Western Blotting.Results Morroniside increased the cell survival rate in 10～100 μmol/L.Compared with control group,MTT metabolic rate(P<0.05),axonal length(P<0.001),area of cell body(P<0.01),cell counting(P<0.05)increased in morroniside-treated group after SH-SY5Y cells were incubated with Morroniside in 10 μmol/L.The expression of NGF in morroniside-treated group was higher than that of the control group after injury induced by H2O2.Conclusion Morroniside shows the neurotrophic effect and can protect SH-SY5Y cells from injury induced by H2O2.

Key words: Morroniside, neurocyte, neurotrophy, neuroprotection