《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (09): 848-849.

• 基础研究 • 上一篇    下一篇

创伤性脑损伤对大鼠海马胶质细胞源性神经营养因子及其受体表达的影响

陈宝友1a; 侯志勇1a; 王志宏1b   

  1. 1.武警医学院附属医院a.脑系科中心;b.科研科,天津市 300162
  • 收稿日期:2007-04-02 出版日期:2007-09-01 发布日期:2007-09-01

Effect of Traumatic Brain Injury on Expression of Glial Cell Line-Derived Neurotrophic Factor and Its Receptors in Hippocampus of Rats

CHEN Bao-you, HOU Zhi-yong, WANG Zhi-hong   

  1. Center of Neurosurgery, Medical College of the People's Armed Police Force, Tianjin 300162, China
  • Received:2007-04-02 Published:2007-09-01 Online:2007-09-01

PDF (PC)

被引次数

9

摘要： 目的研究创伤性脑损伤对大鼠海马胶质细胞源性神经营养因子(GDNF)及其受体(GFRα-1、Ret)表达的影响。方法复制Marmarou's大鼠落体打击损伤模型,将大鼠随机分为正常组、假手术组及损伤后1h组、2h组、4h组、8h组、12h组、24h组、48h组、72h组和5d组。制备组织芯片,采用免疫组化法检测海马GDNF、GFRα-1、Ret的表达情况。结果正常组、假手术组海马中可见到GDNF及其受体低水平表达,损伤后2h海马GDNF的表达达到高峰,损伤后72h降至正常水平;GFRα-1和Ret的表达在损伤后4h达到高峰,以后逐渐下降。结论大鼠创伤性脑损伤后海马GDNF及其受体在早期即明显表达,两者在脑损伤后表达的时序性变化特点基本一致,共同参与创伤性脑创伤的病理生理过程。

关键词: 创伤性脑损伤, 胶质细胞源性神经营养因子(GDNF), 受体, 海马, 大鼠

Abstract: Objective To study the effect of traumatic brain injury on the expression of glial cell line-derived neurotrophic factor(GDNF)and its receptors in hippocampus of rats.Methods Male Sprague-Dawley rats were divided into normal control,sham group and injury groups.The rats of injury groups were subjected to Marmarou's closed traumatic brain injury and then were subdivided into 1 h,2 h,4 h,8 h,12 h,24 h,48 h,72 h and 5 d groups according to the time elapsed after injury.The expression of GDNF and its receptors were measured with immunohistochemistry.Results Mild expression of GDNF and its receptors were observed in hippocampus of rats in control group.The number of GDNF positive neurons reached the peak level in hippocampus 2 h after injury,and that of GFRα-1 and Ret positive neurons reached the peak level 4 h after injury.Conclusion The expression of GDNF and its receptors were increased significantly at the early time in hippocampus of rats after injury in a similar temporal patterns after brain injury.

Key words: traumatic brain injury, glial cell line-derived neurotrophic factor, receptor, hippocampus, rats