《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (09): 853-855.

• 基础研究 • 上一篇    下一篇

剪切力对内皮细胞及其与小肠黏膜下基质复合培养的形态学影响

冷晔1; 丁祖泉1; 高艳琴1; 徐根林2   

  1. 1.同济大学生命科学与技术学院,上海市 200092;2.上海市医疗器械专科学校,上海市 200093
  • 收稿日期:2007-04-05 出版日期:2007-09-01 发布日期:2007-09-01

Morphological Influence of Shear Stress on Endothelial Cells Co-cultured with or without Small Intestinal Submucosa

LENG Ye,DING Zu-quan, GAO Yan-qin, et al   

  1. School of Life Science and Technology, Tongji University, Shanghai 200092, China
  • Received:2007-04-05 Published:2007-09-01 Online:2007-09-01

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摘要： 目的探讨切变应力对猪髋动脉内皮细胞单独培养和猪髋动脉内皮细胞与猪小肠黏膜下基质联合培养形态学特征的影响。方法对猪髋动脉内皮细胞单纯培养组,小肠黏膜下基质与猪髋动脉内皮细胞联合培养组均施与40×10-5N/cm2的剪切应力,持续12h,两组均每隔30min记录细胞图像,计算定向角。结果单纯培养组:第1小时可见有明显的细胞脱落,细胞形态变化不明显;第4小时,细胞脱落已不明显,细胞由多角形逐渐变圆;第8小时,看不到明显的细胞脱落,细胞由第4小时时的类圆形向长梭形发展,细胞变得细长,局部可见细胞沿流场方向排列;第12小时,细胞变得更加细长,细胞沿流场方向排列趋势更为明显;定向角第12小时时与剪切前相比有显著性差异。联合培养组:第1小时有少数细胞脱落,但不如单纯培养组第1小时细胞脱落明显;第2小时后看不到明显的细胞脱落;定向角在12h内未发生显著性变化。结论40×10-5N/cm2的脉动剪切力对猪髋动脉细胞的形态和排列在12h内产生影响;但对与猪小肠黏膜下基质复合培养的内皮细胞影响不大。

关键词: 剪切力, 小肠黏膜下基质, 内皮细胞, 组织工程

Abstract: Objective To investigate the morphological effect of fluid shear stress on pig iliac endothelium cells cultured solely or co-cultured with pig small intestinal submucosa.Methods The shear stress of 40×10-5 N/cm2 were carried out for 12 h on both groups.The images were recorded every 30 min.The directional angles were calculated.Results In the group of cell cultured solely:The defluvium of cells was obvious at the 1st hour,but the shape of cells didn't change.At the 4th hour,the defluvium of cells was little,the cell became round from its initiatory polygon shape.At the 8th hour,the defluvium of cell could not be observed.The shape of cells became fusiform and gracile.The cells arranged along the direction of flow field in the local area.At the 12th hour,the cells became more and more gracile.The trend of realignment of cells along the direction of flow field was obviously.The directional angles of cells at the 12th hour was significantly different from the zero hour.In the group of cell co-cultured with small intestinal submucosa:At the 1st hour,some of cells were brushed off mildly.The defluvium of cells could not been observed since the 2nd hour.The directional angles didn't change significantly in the 12 hours.Conclusion The shear stress of 40×10-55 N/cm2 cannot influence the cell of co-cultured but do influence the cell cultured solely.

Key words: shear stress, small intestinal submucosa, endothelium cells, tissue engineering