《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (10): 928-930.

• 基础研究 • 上一篇    下一篇

亚低温对胆红素脑病仔鼠脑保护作用的实验研究

李金玲; 李林; 李翠玲; 谭红香   

  1. 广州市儿童医院神经康复科; 广州市儿童医院神经康复科 广东广州市; 广东广州市
  • 收稿日期:2007-08-07 出版日期:2007-10-01 发布日期:2007-10-01

Protective Effect of Mild-hypothermia on Brain of Newborn Rats with Bilirubin Encephalopathy

LI Jin-ling, LI Lin, LI Cui-ling, et al   

  1. The Department of Rehabilitation, the Children Hospital of Guangzhou City, Guangzhou 510120, Guangdong, China
  • Received:2007-08-07 Published:2007-10-01 Online:2007-10-01

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被引次数

2

摘要： 目的观察胆红素脑病仔鼠血液神经元特异性烯醇化酶(NSE)、S-100蛋白(S-100β)、总胆红素含量(TBC)的变化及亚低温的脑保护作用。方法出生7 d的Wistar仔鼠42只随机分为C组(对照组,n=10)、M0组(未干预组,n=17)和M1组(亚低温组,n=15);C组给予腹腔注射生理盐水0.5 ml,其余各组腹腔注射胆红素200 mg/kg制成胆红素脑病模型。通过病理和血液NSE、S-100β值的变化,观察亚低温对胆红素脑病仔鼠的脑保护作用。结果胆红素脑病新生仔鼠出现明显的神经行为异常和病理改变,M1组经亚低温干预后血液NSE、S-100β、TBC下降,与M0组相比差异有非常显著性意义(P<0.01)。结论亚低温对胆红素脑病仔鼠有脑保护作用。

关键词: 胆红素脑病, 亚低温, 神经元特异性烯醇化酶, S-100蛋白, 新生鼠

Abstract: Objective To observe the changes of neuron-specific enolase(NSE),S-100 protein and TBC in the blood of newborn rats at early stage of bilirubin encephalopathy,and the protective effect of mild-hypothermia on the brain.Methods 42 Wistar rats(7-day postnatal) were divided randomly into the group C(control group,n=10),group M0(normal-temperature group,n=17) and group M1(mild-hypothermia group,n=15).The rats of the group C received physiological saline 0.5 ml,the rats in the groups of M0 and M1 were injected with bilirubin intraperitoneally(200 mg/kg) to establish the model of bilirubin encephalopathy.The changes of the content of NSE and S-100 protein in the blood of newborn rats,and the protective effect of mild-hypothermia on brain were observed.Results The animals with established bilirubin encephalopathy shown significant changes of neurobehaviour and pathological examination.Values of NSE and S-100 protein of the group M1 decreased after the treatment of mild-hypothermia,and there was a significant difference compared with group M0( P<0.01).Conclusion The mild-hypothermia has protective effect on brain of newborn rats with bilirubin intraperitoneally.

Key words: bilirubin encephalopathy, mild-hypothermia, neuron-specific enolase(NSE), S-100 protein, newborn rat