《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (10): 930-932.

• 基础研究 • 上一篇    下一篇

聚ADP核糖聚合酶1在人类乳腺癌中的表达及其与临床预后相关因子的关系

孔萌萌; 陆东东; 陈宇星   

  1. 同济大学医学部生命科学与技术学院,上海市 200092
  • 收稿日期:2007-06-06 出版日期:2007-10-01 发布日期:2007-10-01

Expression of Poly ADP-Ribose Polymerase-1 in Human Breast Cancer Tissue and Its Relationship with Prognosis Related Factors

KONG Meng-meng, LU Dong-dong, CHEN Yu-xing   

  1. School of Life Science and Technology, Tongji University, Shanghai 200092, China
  • Received:2007-06-06 Published:2007-10-01 Online:2007-10-01

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摘要： 目的研究聚ADP核糖聚合酶1(PARP-1)在人类乳腺癌中的表达及其与患者临床预后相关因子间的相关性。方法采用RT-PCR及免疫荧光染色技术,检测66例手术切除的人类乳腺癌组织及癌旁组织中PARP-1的表达,并分析乳腺癌患者的康复相关因子与PARP-1表达间的关系。结果与癌旁组织相比,94.9%(62/66)的癌组织中PARP-1 mRNA表达上调,90.9%(60/66)的癌组织中PARP-1蛋白表达上调。临床分期越晚、肿瘤分化程度越差,PARP-1的标记指数(LI)、表达指数(EI)越大;单纯癌、浸润癌患者中PARP-1的LI、EI值较大;乳腺癌转移患者的PARP-1的LI、EI值显著高于非转移患者。结论PARP-1基因在转录和翻译水平上的过表达与乳腺癌的分期、病理分级、病理类型、肿瘤转移等临床预后相关因子有关。

关键词: 聚ADP核糖聚合酶1(PARP-1), 乳腺癌, 预后

Abstract: Objective To investigate the expression of poly ADP-ribose polymerase-1(PARP-1) in human breast cancer tissue and its relationship with patient's prognosis related factors.Methods The expression of PARP-1 in breast cancer tissues and their adjacent non-cancerous tissues from resection in 66 operated cases were detected using RT-PCR and immunofluorecence staining(IFS).The relationship between the expression of PARP-1 and the prognosis related factors was analyzed.Results Compared with that of the adjacent non-cancerous tissues,the expression of PARP-1 mRNA increased in 94.9%(62/66) patients,while the expression of PARP-1 protein increased in 90.9%(60/66) ones.The label index(LI) and the expression index(EI) of PARP-1 gene increased especially in the cases in later clinical stage,with poor tumor differentiation grade,of pure breast carcinomas or infiltrative breast carcinomas and of metastasis.Conclusion The expression of PARP-1 in human breast cancer tissue on the level of transcription and translation increases,which may be positively related with the patient's prognosis.

Key words: poly ADP-ribose polymerase-1(PARP-1), breast cancer, prognosis