《中国康复理论与实践》

《中国康复理论与实践》 ›› 2007, Vol. 13 ›› Issue (11): 1023-1025.

• 基础研究 • 上一篇    下一篇

莫诺苷抑制过氧化氢诱导的SH-SY5Y神经细胞氧化损伤

艾厚喜1; 黄文婷2; 王文1; 李林1; 安宜2   

  1. 1.首都医科大学宣武医院药物研究室,教育部神经变性病学重点实验室,北京市 100053;2.北京理工大学生命科学与技术学院生物技术系,北京市 100053
  • 收稿日期:2007-05-13 出版日期:2007-11-01 发布日期:2007-11-01

Morroniside Inhibits H_2O_2-Induced Oxidative Injury in SH-SY5Y Neuroblastoma Cells

AI Hou-xi,HUANG Wen-ting,WANG Wen,et al   

  1. Department of Pharmacology, Xuanwu Hospital of Capital Medical University;Key Laboratory for Neurodegenerative Disease of Ministry of Education, Beijing 100053, China
  • Received:2007-05-13 Published:2007-11-01 Online:2007-11-01

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摘要： 目的研究莫诺苷对H2O2诱导的神经细胞氧化损伤的影响。方法培养的人神经母细胞瘤细胞系SH-SY5Y细胞加入莫诺苷(1、10、100μmol/L)预孵育24h,加入H2O2500μmol/L作用18h诱导产生氧化损伤,测定细胞内活性氧(ROS)、一氧化氮(NO)和谷胱甘肽(GSH)含量。结果莫诺苷(10、100μmol/L)使活性氧的释放分别降低了37%(P<0.01)、27%(P<0.05),NO的释放分别降低了31%(P<0.05)、53%(P<0.01),莫诺苷(1、10、100μmol/L)有明显抑制GSH的降低的作用。结论莫诺苷能够抑制H2O2诱导的SH-SY5Y细胞氧化损伤,有神经保护作用。

关键词: 莫诺苷, 神经细胞, 氧化应激, 一氧化氮, 谷胱甘肽

Abstract: Objective To investigate the effects of Morroniside on H2O2-induced oxidative injury in SH-SY5Y neuroblastoma cells.MethodsSH-SY5Y cells were pre-incubated with Morroniside(1,10,and 100 μmol/L)for 24 h prior to exposure to H2O2(500 μmol/L)for 18 h.The content of reactive oxygen species(ROS),nitric oxide(NO),glutathione(GSH)were determined.ResultsPretreatment the cells with Morroniside(10 and 100 μmol/L)lowered H2O2-induced ROS release by 37%(P<0.01)and 27%(P<0.05)versus H2O2,and NO release in a dose-dependent manner by 31%(P<0.05),53%(P<0.01)versus H2O2.Morroniside(1,10,and 100 μmol/L)increased GSH level in cells treated with H2O2 in comparison with cells exposed only to H2O2,respectively.ConclusionMorroniside shows neuroprotection effect against H2O2-induced oxidation injury in SH-SY5Y cell.

Key words: Morroniside, neurocyte, oxidative stress, nitric oxide, glutathione