《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (01): 24-25.

• 基础研究 • 上一篇    下一篇

胶质细胞源性神经营养因子及其受体在大鼠创伤性脑损伤后皮层中的表达

陈宝友; 侯志勇   

  1. 武警医学院附属医院脑系科中心,天津市 300162
  • 收稿日期:2007-10-10 出版日期:2008-01-01 发布日期:2008-01-01

Expressions of Glial Cell Line-derived Neurotrophic Factor and Its Receptors in Cortex of Rats after Closed Traumatic Cerebral Injury

CHEN Bao-you, HOU Zhi-yong.   

  1. Center of Neurosurgery, Medical College of the People's Armed Police Force, Tianjin 300162, China
  • Received:2007-10-10 Published:2008-01-01 Online:2008-01-01

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被引次数

18

摘要： 目的探讨大鼠闭合性创伤性脑损伤后不同时间胶质细胞源性神经营养因子(GDNF)及其受体GFRα-1、Ret在大脑皮层的表达。方法制备Marmarou's大鼠落体打击损伤模型,将大鼠随机分为正常对照组、手术对照组及损伤后1h组、2h组、4h组、8h组、12h组、24h组、48h组、72h组和5d组。制备组织芯片,采用免疫组化法检测大脑皮层GDNF、GFRα-1、Ret的表达。结果正常组、手术对照组皮层中可见GDNF及其受体低水平表达,损伤后2h,皮层中GDNF的表达达到高峰,大量表达可持续至伤后5d,GFRα-1和Ret的表达在损伤后4h时达到高峰,于伤后24h降至正常。结论大鼠闭合性创伤性脑损伤后皮层中GDNF及其受体在早期即明显表达,两者在脑损伤后表达的时序性变化特点基本一致,共同参与创伤性脑创伤的病理生理过程。

关键词: 创伤性脑损伤, 胶质细胞源性神经营养因子(GDNF), 受体, 大鼠

Abstract: Objective To investigate the expressions of glial cell line-derived neurotrophic factor(GDNF)and its receptors,GFRα-1 and Ret,in cortex of rats after closed traumatic cerebral injury.Methods Male Sprague-Dawley rats were divided into normal control,sham and injury groups.The rats of injury groups were subjected to Marmarou's closed traumatic cerebral injury and then were subdivided into 1 h,2 h,4 h,8 h,12 h,24 h,48 h,72 h and 5 d groups according to the time elapsed after injury.The expression of GDNF and its receptors were determined with immunohistochemistry.Results Mild expression of GDNF and its receptors were observed in cortex of rats in control groups.The number of GDNF positive neurons reached the peak level in cortex 2 h after injury,and that of GFRα-1 and Ret positive neurons reached the peak level 4 h after injury.Conclusion The expressions of GDNF and its receptors increased significantly at the early time in cortex of rats after injury,as well as its receptors.It suggests that GDNF and its receptors play an important role after traumatic cerebral injury.

Key words: traumatic cerebral injury, glial cell line-derived neurotrophic factor(GDNF), receptor, rat