《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (02): 164-165.

• 临床研究 • 上一篇    下一篇

蛛网膜下腔植入APA-BCC微胶囊对癌痛患者脑脊液中镇痛相关物质含量的影响

郭水龙; 潘静坤; 高宇红; 崔忻; 罗芸; 田磊; 薛毅珑   

  1. 解放军总医院老年医学研究所细胞生物室,北京市 100853
  • 收稿日期:2007-05-23 出版日期:2008-02-01 发布日期:2008-02-01

Effect of APA Microencapsulized Bovine Chromaffin Cells Transplantation on Endogenous Opioid Peptides and Catecholamine Concentration in CSF of Cancer Pain Patients

GUO Shui-long, PAN Jing-kun, GAO Yu-hong, et al   

  1. Institute of Geriatrics, the General Hospital of PLA, Beijing 100853, China
  • Received:2007-05-23 Published:2008-02-01 Online:2008-02-01

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摘要： 目的观察蛛网膜下腔植入微囊化牛肾上腺嗜铬细胞(APA-BCC)对癌痛患者中枢内源性脑啡肽类和儿茶酚胺类物质含量的影响。方法随机将不同剂量的APA-BCC植入中、重度癌痛患者的腰段脊髓蛛网膜下腔内;应用放射免疫法检测移植前后脑脊液中亮氨酸脑啡肽(L-EK)、β-内啡肽(β-EP)和强啡肽A(DynA)的含量;应用高压液相法检测去甲肾上腺素(NA)和肾上腺素(AD)的含量;应用NRS法计算癌痛患者的疼痛缓解程度。结果移植APA-BCC后,脑脊液中L-EK含量上升,移植剂量为1.0×107和1.25×107个细胞时,L-EK升高最为显著,分别升高155.27%和154.28%;β-EP、DynA、NA和AD含量无明显改变;完全缓解、部分缓解和无效的患者,L-EK分别升高241.07%、130.45%和115.78%。结论APA-BCC植入对癌痛患者的镇痛效应可能与中枢L-EK含量升高有关;1.0×107和1.25×107可能是较佳的细胞移植剂量。

关键词: 移植, 镇痛, 嗜铬细胞

Abstract: Objective To observe the effect of APA-BCC(alginate-polylysine-alginate microencapsulated bovine adrenal medullary chromaffin cells)microcapsules transplantation into the subarachnoid space of cancer pain patients on endogenous opioid peptides and catecholamine concentration in cerebrospinal fluid(CSF).Methods The different doses of APA-BCC microcapsules were transplanted into the spinal subarachnoid space of cancer patients with moderate or serious pain.The concentrations of Leu-enkephalin(L-EK),β-endorphin(β-EP),dynorphin A(DynA),noradrenaline(NA)and adrenaline(AD)in CSF were tested.Results L-EK concentration in CSF increased remarkably after transplantation,and the increase was most remarkable when transplantation doses were 1.0×107and 1.25×107;there were no remarkable changes of β-EP,DynA,NA and AD after transplantation.Conclusion The analgetic effects of APA-BCC microcapsules tranplantation may correlate with the increase of L-EK in CSF of cancer pain patients;the dose of 1.0×107 and 1.25×107cells may be the most effective dose.

Key words: tranplantation, ease pain, chromaffin cell