《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (03): 228-300.

• 基础研究 • 上一篇    下一篇

重复经颅磁刺激对脊髓运动功能恢复的影响及其机制的实验研究

张新1; 李建军1,2; 霍小林3; 戴红4; 潘立东5   

  1. 1.北京博爱医院神经外科,北京市 100068;2.首都医科大学康复医学院,北京市 100068;3.中国科学院电工研究所,北京市 100080;4.首都医科大学康复研究室,北京市 100054;5.中国康复研究中心基础研究所,北京市 100068
  • 收稿日期:2007-11-20 出版日期:2008-03-01 发布日期:2008-03-01

Effects of Repetitive Transcranial Magnetic Stimulation on Locomotor Outcome of Spinal Cord Injured Rats

ZHANG Xin, LI Jian-jun, HUO Xiao-lin, et al   

  1. Departmentof Neurosurgery, BeijingCharity Hospital, Capital Medical University School of Rehabilitation Medicine, China Rehabilitation Research Centre, Beijing 100068, China
  • Received:2007-11-20 Published:2008-03-01 Online:2008-03-01

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被引次数

49

摘要： 目的观察重复经颅磁刺激(rTMS)对大鼠脊髓损伤后运动功能恢复的影响,并对其作用机制进行初步探索。方法利用重物撞击法制作成年大鼠T10脊髓损伤模型,实验组给予经皮0.5Hz大脑皮质区磁刺激,每天500个脉冲,共4周。通过运动行为学BBB评分,组织学及免疫组化荧光法观察生长相关蛋白43(GAP43)和5-羟色胺(5-HT)在脊髓损伤区的变化情况。结果实验组BBB评分明显高于模型组(P<0.01);组织学表现符合慢性不完全性脊髓损伤病理变化;实验组GAP43及5-HT水平明显高于模型组(P<0.01);且损伤头端增加明显著高于尾端(P<0.01)。结论rTMS有促进脊髓损伤大鼠运动功能恢复的作用,机制可能与促进轴突生长锥可塑性及残存下行5-HT能纤维神经递质分泌增加有关。

关键词: 脊髓损伤, 重复经颅磁刺激(rTMS), 生长相关蛋白43(GAP43), 5-羟色胺(5-HT)

Abstract: Objective To explore the effect of repetitive transcranial magnetic stimulation(rTMS)on spinal cord injured rats.Methods Weight-drop spinal cord injury model was made at thoracic 10 segments with NYU impactor device.Stimulated group received daily superthreshold rTMS continued for 4 weeks.BBB locomotor scores were recorded weekly.Growth associated protein 43(GAP43)and 5-hydroxytryptamine(5-HT)were detected with immunofluorescence staining in the area of rostral and caudal to the lesion.Results The BBB scores in stimulation group improved compared with that in the control(P<0.01).GAP43 and 5-HT markers increased in the stimulation group(P<0.01),and they increased in the rostral than in the caudal areas(P<0.01).Conclusion rTMS can improve the locomotor function of incomplete spinal cord injury rats,which may result from the increase of expression of GAP43 and 5-HT.

Key words: spinal cord injury, repetitive transcranial magnetic stimulation(rTMS), growth associated protein 43(GAP43), 5-hydroxytryptamine(5-HT)