《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (03): 239-240.

• 基础研究 • 上一篇    下一篇

茶多酚对帕金森病模型小鼠多巴胺神经元保护作用的实验研究

张扬; 邵蓓   

  1. 温州医学院附属第一医院神经内科,浙江温州市 325000
  • 收稿日期:2007-12-10 出版日期:2008-03-01 发布日期:2008-03-01

Effect of Green Tea Polyphenols on N-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine Induced Dopaminergic Neurodegeneration in Mice

ZHANG Yang, SHAO Bei   

  1. Department of Neurology, The First Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000,Zhejiang, China
  • Received:2007-12-10 Published:2008-03-01 Online:2008-03-01

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摘要： 目的探讨茶多酚对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)致帕金森病模型小鼠多巴胺能神经元的保护作用。方法70只C57BL/6小鼠分为4组,A组为生理盐水(NS)+NS组,B组为NS+茶多酚(GTPs)组,C组(模型组)为NS+MPTP组,D组为GTPs+MPTP组,其中D组又分为D1组(10mg/kg)及D2(50mg/kg)组,在注射GTPs后0.5h腹腔注射MPTP3次,制作帕金森病模型。腹腔注射茶多酚后,应用高效液相色普-电化学分析法(HPLC-ECD)测定多巴胺及其代谢产物浓度。结果A组多巴胺及代谢产物含量最高,B组与A组无显著性差异,C组与A组比较有显著性差异(P<0.05),D组与C组相比浓度升高(P<0.05)。结论茶多酚对MPTP诱发的帕金森病模型小鼠黑质多巴胺神经元有保护作用。

关键词: 茶多酚, 帕金森病, 多巴胺, 小鼠

Abstract: Objective To investigate the protective effects of green tea polyphenols(GTPs)on dopamine neuron loss in substantia nigra concomitant with a depletion of dopamine in corpus striatum induced by the N-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP)in mice as a model of Parkinson disease.Methods Male C57BL/6 mice were intraperitoneally injected with saline + saline(group A),saline + GTPs(group B),saline+MPTP(group C)and GTP+MPTP(group D)at 2-hour intervals for a total of 3 doses for MPTP and 5 doses for GTPs(10 or 50 mg/kg delivered).The animals were sacrificed 7 d after the last injection.Levels of dopamine and its metabolites in the corpus striatum were measured with high-performance liquid chromatography with an electrochemical detector(HPLC-ECD).Results Level of dopamine and its metabolites in the corpus striatum in group C decreased significantly compared with group A or B;however,those in group D(both 10 and 50 mg/kg)prevented these effects.Conclusion GTPs can protect the dopamine neurons from loss in MPTP-induced mice.

Key words: green tea polyphenols(GTPs), Parkinson disease, dopamine, mice