《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (04): 321-322.

• 专题 • 上一篇    下一篇

葛根素对动脉粥样硬化兔髂动脉C反应蛋白表达的影响

胡文志1; 黄翘胜2; 孙烈1; 杨季明1; 洪梅1; 蒋振忠1; 杨富1; 张博晴1; 周海波1   

  1. 1.南京医科大学第二附属医院心血管内科,江苏南京市 210011;2.郧阳医学院附属医院十堰市太和医院,湖北十堰市 442000
  • 收稿日期:2007-11-27 出版日期:2008-04-01 发布日期:2008-04-01

Effect of Puerarin on Expression of C-reactive Protein in Iliac Artery of Atherosclerotic Rabbit

HU Wen-zhi, HUANG Qiao-sheng,SUN Lie, et al   

  1. The Department of Cardiology, Nanjing Medical University Second Hospital, Nanjing 210011, Jiangsu, China
  • Received:2007-11-27 Published:2008-04-01 Online:2008-04-01

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被引次数

2

摘要： 目的观察葛根素对动脉粥样硬化兔髂动脉分泌和表达C反应蛋白(CRP)的影响。方法20只家兔分为正常对照组(6只,正常饮食)、基础对照组(8只,球囊损伤和高脂饮食)和葛根素干预组(8只,球囊损伤、高脂饮食和葛根素干预),球囊损伤后4周处死;取一侧病变髂动脉做病理切片,应用免疫组化法测定CRP的表达;取另一侧病变髂动脉抽提总RNA,应用半定量逆转录多聚酶链式反应(RT-PCR)测定CRP mRNA的表达。结果葛根素干预组髂动脉粥样斑块CRP mRNA及其蛋白质表达均明显低于基础对照组(P<0.01)。结论葛根素可以通过调节兔动脉粥样斑块分泌CRP途径发挥稳定动脉粥样硬化斑块的作用。

关键词: 葛根素, 动脉粥样硬化, C反应蛋白

Abstract: Objective To investigate the effect of Puerarin on expression of C-reactive protein(CRP)in iliac artery of atherosclerotic rabbits.Methods Twenty male New Zealand white rabbits were divided into the normal control group(n=6,fed on a normal diet),basic control group(n=8,fed on a cholesterol diet)and Puerarin group(n=8,fed on a cholesterol diet).The balloon injury of iliac arteries was performed in animals of the basic control group and Puerarin group,and the animals of the Puerarin group were injected with Puerarin(25 mg/kg/d per rabbit)after injury.Four weeks after balloon injury,untreated and balloon injury iliac arteries were harvested for immunocytochemical staining.The mRNA and protein expression of CRP were tested by RT-PCR and immunucytochemistry.Results The mRNA and expression of CRP in atheroseclerotic plaque of iliac artery in the Puerarin group was significantly lower than that in the basic control group(P<0.01).Conclusion Puerarin can make atheroseclerotic plaque stable by regulating of the expression of CRP in iliac artery of atherosclerotic rabbit.

Key words: Puerarin, atherosclerosis, C-reactive protein