《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (04): 326-328.

• 专题 • 上一篇    下一篇

螺内酯对人工高血压大鼠心脏保护作用研究

雒生杰1,2; 刘晓惠3; 万昕红4; 张麟4   

  1. 1.首都医科大学康复医学院,北京市 100068;2中国康复研究中心北京博爱医院,北京市 100068；3.首都医科大学附属北京安贞医院,北京市 100029;4.首都医科大学附属北京朝阳医院,北京市 100020
  • 收稿日期:2007-10-24 出版日期:2008-04-01 发布日期:2008-04-01

Protective Effect of Spironolactone on Heart of Hypertensive SD Rats

LUO Sheng-jie, LIU Xiao-hui, WAN Xin-hong, et al   

  1. Capital Medical University School of Rehabilitation Medicine, BeijingCharity Hospital, China Rehabilitation Research Center, Beijing 100068, China
  • Received:2007-10-24 Published:2008-04-01 Online:2008-04-01

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摘要： 目的观察螺内酯对人工高血压大鼠的心脏保护作用。方法30只SD大鼠随机分为正常对照组、高血压组、小剂量螺内酯组和大剂量螺内酯组,后3组动物采用左旋精氨酸甲酯(L-NAME)50mg/kg/d灌胃,之后高血压组随意饮用1%的盐水,小剂量螺内酯组予螺内酯20mg/kg/d灌胃,大剂量螺内酯组予螺内酯100mg/kg/d灌胃。8周后处死动物取心脏,观察心肌细胞横截面积、心肌小动脉周围纤维面积/小动脉腔面积之比、管壁面积/小动脉腔面积、心肌胶原面积与心肌总面积之比、心肌损害情况。结果高血压组心肌细胞横截面积明显大于其他3组(P<0.01);螺内酯组心肌细胞横截面积明显小于高血压组(P<0.01),与正常对照组相比差异无显著性意义;高血压组心肌胶原含量明显高于其他3组(P<0.01);螺内酯组明显高于正常对照组(P<0.01);高血压组小动脉管壁面积/管腔面积大于其他3组(P<0.05～0.01);正常对照组和螺内酯组间的差异无显著性意义;高血压组心肌可见片状坏死;螺内酯组片状坏死明显较轻;不同剂量螺内酯组间无明显差异。结论螺内酯对高血压大鼠心脏具有明显保护作用。

关键词: 螺内酯, 高血压, 心脏保护

Abstract: Objective To observe the protective effect of spironolactone on heart of hypertensive SD rats.Methods 30 male SD rats were randomly divided into the control group,hypertensive group(HS group),low spironolactone dose group(HSL group)and high spironolactone dose group(HSH group).The animals except the control group were treated with L-NAME 50 mg/kg/d.Then the animals of the HS group were fed with 1% sodium chloride water solution.Those of the HSL and HSH groups were treated same but the former added with spironolactone 20 mg/kg/d,the later added with spironolactone 100 mg/kg/d.8 weeks late,the animals were killed and hearts were taken out to observe following items:myocardial cross-sectional area,ratio of per-arteriolar fibrous area/arteriolar luminal area of arterioles,ratio of collagen area/total myocardial area,myocardial necrosis and inflammation.Results The myocardial cross-sectional areas in the HS group were significantly more than those in other 3 groups(P<0.01).Collagen ratios of the HS group were significantly higher than others(P<0.01).The ratios of arteriolar wall area versus luminal area in the HS group were significantly higher than those of control and HSH groups(P<0.01).Myocardial injuries in the HS group were more severe than those of other 3 groups.There were no differences between HSL and HSH groups.Conclusion Spironolactone has protective effect on heart of hypertensive SD rats.

Key words: spironolactone, hypertension, cardio protection