《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (04): 338-340.

• 基础研究 • 上一篇    下一篇

二苯乙烯苷对过度表达α-synuclein蛋白的转基因COS-7细胞株的影响

刘莹1; 李林1; 杨慧2; 李雅莉1; 张兰1   

  1. 1.首都医科大学宣武医院药物研究室,神经变性病教育部重点实验室,北京市 100053;2.首都医科大学北京神经科学研究所,北京市 100069
  • 收稿日期:2008-02-14 出版日期:2008-04-01 发布日期:2008-04-01

Effects of Tetrahydroxy-stilbene Glycoside on Over-expression of α-synuclein Protein in Transgenic COS-7 Cells

LIU Ying, LI Lin,YANG Hui, et al   

  1. The Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China
  • Received:2008-02-14 Published:2008-04-01 Online:2008-04-01

PDF (PC)

被引次数

6

摘要： 目的观察二苯乙烯苷(TSG)在体外试验中对COS-7细胞株过度表达α-synuclein蛋白的影响。方法将不同浓度的TSG与转染α-synuclein基因的COS-7细胞共孵育24h,应用二甲基噻唑蓝(MTT)法检测细胞存活率,免疫细胞化学和Western免疫印迹法检测α-synuclein蛋白的表达,观察TSG对其的影响。结果TSG在12.5～200.0μmol/L浓度范围内与COS-7细胞孵育24h不影响细胞存活率,而在免疫细胞化学和Western免疫印迹分析中均显示TSG剂量依赖性地抑制α-synuclein蛋白表达和聚集。结论TSG在体外对COS-7细胞过度表达α-synuclein有明显抑制作用。

关键词: 二苯乙烯苷, 何首乌, α-synuclein, COS-7细胞, 体外试验

Abstract: Objective To explore the effects of 2,3,5,4'-tetrahydroxy stilbene-2-β-D-glycoside(TSG)on the over-expression and aggregation of α-synuclein in vitro.Methods TSG in different concentrations was incubated with α-synuclein transgenic COS-7 cells for 24 h.The cell viability was measured by MTT method.The expression of α-synuclein protein was determined by immunocytochemistry and Western blotting method.Results Incubation of TSG at the range of 12.5～200.0 μmol/L with α-synuclein transgenic COS-7 cells for 24 h did not influence cell viability,but a dose-dependently inhibition for the over-expression of α-synuclein protein could be observed in the tests of immunocytochemistry and Western blotting.Conclusion TSG can inhibit the over-expression of α-synuclein protein in COS-7 cells in vitro.

Key words: 4'-tetrahydroxy stilbene-2-β-D-glycoside(TSG), Polygonum multiflorum, α-synuclein, COS-7 cell, in vitro