《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (04): 341-342.

• 基础研究 • 上一篇    下一篇

绿色荧光蛋白标记的大鼠神经干细胞移植于损伤脊髓后的迁移和存活

高川川1,2; 夏雷2; 郝淑煜2; 历俊华2; 万虹2   

  1. 1.首都医科大学2005级研究生,北京市 100054;2.首都医科大学北京市神经外科研究所,北京市 100050
  • 收稿日期:2008-01-22 出版日期:2008-04-01 发布日期:2008-04-01

Migration and Survival of Rat Neural Stem Cells Marked by Green Fluorescent Protein after Transplanted to Injured Spinal Cord

GAO Chuan-chuan, XIA Lei, HAO Shu-yu, et al.   

  1. Postgraduate School of Capital Medical University, Beijing 100054, China
  • Received:2008-01-22 Published:2008-04-01 Online:2008-04-01

PDF (PC)

被引次数

13

摘要： 目的观察神经干细胞在体内的迁移和存活。方法体外培养胎鼠脊髓神经干细胞,用表达绿色荧光蛋白(GFP)的慢病毒载体进行转染,以乙交酯-丙交酯共聚物(PLGA)为支架移植入大鼠T9半横断脊髓损伤处。移植后1个月在荧光显微镜下观察神经干细胞在脊髓内的迁移,并计算其存活率。结果神经干细胞表达强烈的绿色荧光。细胞移植后1个月,在损伤脊髓的头端和尾端都可见GFP阳性细胞,计算出的存活率为(1.4911±0.0313)%。结论GFP标记的神经干细胞移植入大鼠损伤脊髓后向脊髓组织内迁移并有少数存活。

关键词: 绿色荧光蛋白, 慢病毒载体, 神经干细胞

Abstract: Objective To investigate the migration and survival of neural stem cells(NSCs)in vivo.Methods NSCs cultured in vitro were transfected by lentiviral vectors expressing green fluorescent protein(GFP)to construct GFP-NSCs,then trans-seeded into lactide-co-glycolide(PLGA)scaffold and implanted into the injured site of T9 spinal cord in rat.One month after transplantation,the migration of NSCs in spinal cord was examined by fluorescence microscope,and the survival rate of NSCs was counted out.Results NSCs labeled GFP had strong expression of green fluorescence.One month after transplanting,part of NSCs expressing GFP could be seen in PLGA scaffolds and rostral,caudal spinal core.The survival rate counted out was 1.4911±0.0313%.Conclusion NSCs marked by GFP and transplanted to rat injured spinal cord could migrate into the spinal cord tissues and the minority of them could survive.

Key words: green fluorescent protein, lentiviral vectors, neural stem cells