《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (04): 349-351.

• 基础研究 • 上一篇    下一篇

脑胶质瘤中P170、TOPⅡ表达与其增殖活性关系的研究

孙淑清; 李桂林; 苏玉金; 徐作霖; 崔云; 方静宜; 李敬军   

  1. 北京市神经外科研究所,北京市 100050
  • 收稿日期:2008-01-08 出版日期:2008-04-01 发布日期:2008-04-01

Correlation of Expressions of P170,TOPⅡ and Proliferation in Gliomas

SUN Shu-qing, LI Gui-lin, SU Yu-jin, et al.   

  1. Beijing Neurosurgical Institute, Beijing 100050, China
  • Received:2008-01-08 Published:2008-04-01 Online:2008-04-01

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摘要： 目的探讨人脑胶质瘤组织中多药耐药基因(P170)、拓扑异构酶Ⅱ(TOPⅡ)的表达与其增殖活性的相关性。方法以核抗原(Ki-67)作为增殖指标,应用单克隆抗体、免疫组化技术S-P法观察54例低级别(Ⅰ～Ⅱ级)和54例高级别(Ⅲ～Ⅳ级)胶质瘤组织中P170、TOPⅡ和Ki-67的表达,并对临床资料进行随访及分析。结果P170、TOPⅡ和Ki-67蛋白在低级别和高级别胶质瘤之间的表达差异均有非常显著性意义(P<0.01);相关分析显示,在胶质瘤中,P170与Ki-67的表达呈负相关(r=-0.276,P=0.019),TOPⅡ与Ki-67的表达呈显著正相关(r=0.637,P<0.001),P170与TOPⅡ的表达之间无相关性。结论胶质瘤中P170、TOPⅡ的表达与其恶性进展相关。

关键词: 胶质细胞瘤, P170(多药耐药基因), TOPⅡ(拓扑异构酶), Ki-67(核抗原)

Abstract: Objective To investigate the correlation of the expressions of P170,topoisomerase Ⅱ(TOPⅡ)and the proliferation in gliomas.Methods Ki-67 was used as the marker of the proliferation in gliomas.The expressions of P170,TOPⅡ and Ki-67 were tested by S-P immunohistochemical technique using monoclonal antibody to their protein in 54 cases of low-grade gliomas(WHO Ⅰ～Ⅱ grade)and 54 cases of high-grade gliomas(WHO Ⅲ～Ⅳ grade),analysis of the clinical and follow-up were performed.Results The expressions of P170,TOPⅡ and Ki-67 had significant differences between low-grade and high-grade gliomas(P<0.01).Correlation analysis showed that the expression of P170 was negatively related with Ki-67(r=-0.276,P=0.019);the expression of TOPⅡ was positively related with Ki-67(r= 0.637,P<0.001);there was no correlation between the expressions of P170 and TOPⅡ.Conclusion The expressions of P170 and TOPⅡ are associated with the malignant prognosis in gliomas.

Key words: gliomas, P170, topoisomerase Ⅱ(TOPⅡ), Ki-67