《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (05): 430-432.

• 基础研究 • 上一篇    下一篇

丹参酮ⅡA对鼠脑缺血再灌注损伤一氧化氮及一氧化氮合酶的影响

李浩; 刘开祥; 俸军林   

  1. 桂林医学院附属医院神经内科,广西桂林市 541002
  • 收稿日期:2007-11-01 出版日期:2008-05-01 发布日期:2008-05-01

Effect of Tanshinone ⅡA on Contents of Nitrous Oxide and Activities of Nitric Oxide Synthase Following Cerebral Ischemic Reperfusion Injury in Rats

LI Hao, LIU Kai-xiang, FENG Jun-lin.   

  1. Departmentof Neurology, the Affiliated Hospital of Guilin Medical College, Guilin 541002, Guangxi, China
  • Received:2007-11-01 Published:2008-05-01 Online:2008-05-01

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被引次数

39

摘要： 目的探讨丹参酮ⅡA(TanⅡA)对脑缺血再灌注损伤大鼠脑组织及血清中一氧化氮(NO)含量、一氧化氮合酶(NOS)及诱导型一氧化氮合酶(iNOS)活性的影响。方法40只Wistar大鼠随机分为假手术组、缺血再灌注组、TanⅡA低剂量治疗组和TanⅡA高剂量治疗组,线栓法建立局灶性脑缺血再灌注模型。TanⅡA高、低剂量治疗组于术前连续灌胃给予高、低剂量TanⅡA3d,每天1次。各组于脑缺血90min再灌注24h进行HE染色观察病理形态学变化,检测脑组织和血清中NO含量和NOS、iNOS活性。结果TanⅡA高、低剂量治疗组脑组织缺血损伤病理学改变轻于缺血再灌注组,TanⅡA高剂量治疗组缺血改变轻于低剂量治疗组。与假手术组比较,缺血再灌注组脑组织和血清中NO含量和NOS、iNOS活性明显升高;与缺血再灌注组比较,TanⅡA高、低剂量治疗组脑组织和血清中NO含量和NOS、iNOS活性均降低,高、低剂量组之间有显著性差异(P<0.05)。结论TanⅡA可减轻神经元损伤程度,对缺血再灌注脑损伤具有保护作用,其机制可能与可降低NOS、iNOS活性,减少NO含量有关。

关键词: 缺血再灌注, 一氧化氮(NO), 一氧化氮合酶(NOS), 诱导型一氧化氮合酶(iNOS), 丹参酮ⅡA

Abstract: Objective To study the effect of Tanshinone ⅡA(Tan ⅡA) on the contents of nitrous oxide(NO) and the activities of nitric oxide synthase(NOS) and immunologic NOS(iNOS) following cerebral ischemia reperfusion(I/R)injury in rats.Methods 40 Wistar rats were randomly divided into 4 groups,which were sham group,I/R group,low dosage Tan ⅡA treated group and high dosage Tan ⅡA treated group.The focal middle cerebral artery occlusion(MCAO) model was made.Rats were pretreated with Tan ⅡA for 3 d respectively before MCAO.After 90 min MCAO following 24 h of reperfusion,HE staining was investigated.The contents of NO and the activities of NOS and iNOS were also investigated.Results The change of ischemic impairment in low or high dosage Tan ⅡA treated group was lighter than that of I/R group,and high dosage Tan ⅡA treated group was lighter than that of low dosage Tan ⅡA treated group.Compared with the sham group,the contents of NO and the activities of NOS and iNOS increased in the ischemic territory(P<0.05).Compared with I/R group,low and high dose Tan ⅡA treated group reduced the contents of NO and the activities of NOS and iNOS dose-dependently(P<0.05).Conclusion Tan ⅡA may reduced cerebral ischemia-reperfusion injure by reducing the contents of NO and the activities of NOS and iNOS dose-dependently.

Key words: ischemia-reperfusion, nitrous oxide(NO), nitric oxide synthase(NOS), immunologic NOS(iNOS), Tanshinone ⅡA