《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (05): 437-439.

• 基础研究 • 上一篇    下一篇

甲基汞对原代大鼠星形胶质细胞的毒性作用

陈茹1; 蔡激扬2   

  1. 同济大学生命科学与技术学院,上海市 200092;2.Vanderbilt Eye Institute Vanderbilt University Medical Center; Nash-ville; TN37232; United States
  • 收稿日期:2008-01-31 出版日期:2008-05-01 发布日期:2008-05-01

Toxicity of Methylmercury on Primary Cultured Rat Astrocyte

CHEN Ru, CAI Ji-yang   

  1. Bioscience and Technology School,Tongji University, Shanghai 200092, China
  • Received:2008-01-31 Published:2008-05-01 Online:2008-05-01

PDF (PC)

被引次数

30

摘要： 目的初步研究甲基汞对原代SD大鼠星形胶质细胞产生毒性的机制。方法用甲基汞作用于原代SD大鼠星形胶质细胞,用高效液相色谱检测细胞氧化还原水平,用Western-blot检测核因子E2相关因子2(Nrf2)蛋白表达水平。结果随着甲基汞浓度的增加,星形胶质细胞的还原水平明显下降。在甲基汞浓度较小时,随着甲基汞浓度的增加,星形胶质细胞Nrf2表达水平呈增长趋势;在甲基汞浓度较大时,随着甲基汞浓度的增加,星形胶质细胞Nrf2表达水平呈减少趋势。结论甲基汞对原代SD大鼠星形胶质细胞的毒性作用可能通过改变还原型谷胱甘肽(GSH)和Nrf2的功能而实现。

关键词: 甲基汞, 星形胶质细胞, 毒性作用, 核因子E2相关因子2(Nrf2)

Abstract: Objective To study the mechanism of toxicity of methylmercury on primary cultured SD rat astrocyte.Methods Treat primary cultured SD rat astrocyte with methylmercury.Determine the level of cyto-oxidation redoxin with High Performance Liquid Chromatography(HPLC).Western-blot was applied to examine the protein expression of nuclear factor erythroid 2 related factor 2(Nrf2).Results With the increase of the concentration of methylmercury,the redoxin level of astrocyte was apparently decreasing.When the concentration of methylmercury was at a low level,the expression of Nrf2 was increasing with the increase of the methylmercury concentration.When the concentration of methylmercury was at a high level,the expression of Nrf2 was decreasing with the increase of the methylmercury concentration.Conclusion The toxicity of methylmercury on primary cultured SD rat astrocyte can be realized through altering the function of GSH and Nrf2.

Key words: methylmercury, astrocyte, toxicity, nuclear factor erythroid-2 related factor 2(Nrf2)