《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (05): 442-444.

• 基础研究 • 上一篇    下一篇

针对人c-Met基因的RNA干扰对晶状体上皮细胞增殖的影响

胡丽婷1; 赵桂秋1; 杜兆东2; 张晶1; 姜楠1; 车成业1   

  1. 1.青岛大学医学院附属医院眼科,山东青岛市 266003;2.北京大学第三医院北京大学眼科中心,山东青岛市 100083
  • 收稿日期:2007-11-13 出版日期:2008-05-01 发布日期:2008-05-01

RNA Interference Targeting c-Met Inhibits Proliferation of Lens Epithelial Cells

HU Li-ting, ZHAO Gui-qiu, DU Zhao-dong, et al   

  1. The Department of Ophthalmology, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003,Shandong, China
  • Received:2007-11-13 Published:2008-05-01 Online:2008-05-01

PDF (PC)

被引次数

4

摘要： 目的探讨针对肝细胞生长因子(HGF)受体c-Met的RNA干扰对人晶状体上皮细胞增殖的影响。方法构建针对人c-Met基因的小干扰RNA(siRNA),转染体外培养的人晶状体上皮细胞,利用实时荧光定量聚合酶链反应(Real-TimePCR)检测细胞中c-Met mRNA的表达变化,应用MTT法检测HGF诱导后人晶状体上皮细胞增殖。结果与对照组相比,转染后的人晶状体上皮细胞中c-Met mRNA表达水平明显下降(P<0.01);与单纯HGF诱导组相比,转染后HGF诱导的人晶状体上皮细胞生长明显减慢,增殖能力明显下降(P<0.01)。结论针对人c-Met基因的RNA干扰可以有效阻断人晶状体上皮细胞中c-Met mRNA的表达,并能抑制HGF诱导的人晶状体上皮细胞的增殖。

关键词: 晶状体, 上皮细胞, 肝细胞生长因子(HGF), c-Met, 小干扰RNA(siRNA)

Abstract: Objective To observe the effect of small-interfering RNA(siRNA) targeting c-Met,the receptor of hepatocyte growth factor(HGF),on the proliferation of human lens epithelial cells(LECs).Methods siRNA was transferred into LECs cultured in vitro by HiperFect Transfection Reagent.Real-Time PCR was applied to observe the expression of c-Met mRNA in LECs after gene transfer,and MTT assay was used to detect the proliferation of LECs induced by HGF.Results The expression of c-Met mRNA in LECs was significantly decreased in the experimental group,compared to that in the controls(P<0.01).Proliferation of LECs induced by HGF was inhibited,compared with the single HGF stimulated group(P<0.01).Conclusion The RNA interference targeting c-Met can effectively inhibit the expression of c-Met mRNA,and the proliferation of LECs induced by HGF.

Key words: lens, epithelial cells, hepatocyte growth factor(HGF), c-Met, small-interfering RNA(siRNA)