《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (06): 524-525.

• 基础研究 • 上一篇    下一篇

慢性脑缺血对老龄大鼠学习记忆能力和星形胶质细胞的影响

常文广1; 滕军放2   

  1. 1.新乡市中心医院神经内科三病区,河南新乡市 453000;2.郑州大学第一附属医院神经内科,河南郑州市 450052
  • 收稿日期:2007-09-13 出版日期:2008-06-01 发布日期:2008-06-01

Effect of Chronic Cerebral Hypoperfusion on Abilities of Learning and Memory and Astrocytes in Aged Rats

CHANG Wen-guang,TENG Jun-fang   

  1. The Department of Neurology, the Center Hospital of Xinxiang, Xinxiang 453000, Henan, China
  • Received:2007-09-13 Published:2008-06-01 Online:2008-06-01

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摘要： 目的观察慢性脑缺血对老龄大鼠学习记忆能力和星形胶质细胞的影响。方法50只Wistar健康老龄大鼠随机分为假手术组和模型组,采用Morris水迷宫检测各组大鼠学习记忆能力的变化;采用免疫组化法观察胶质原纤维酸性蛋白(GFAP)在各组大鼠额叶皮质和海马的表达。结果与假手术组比较,模型组大鼠的学习记忆功能明显下降,GFAP标记的星形胶质细胞大量增生、肥大。结论慢性脑缺血的病理机制中涉及星形胶质细胞的增殖和形态改变,且可能与学习记忆能力下降有关。

关键词: 脑缺血, 学习记忆, 胶质原纤维酸性蛋白, 大鼠

Abstract: Objective To observe the effect of chronic cerebral hypoperfusion on abilities of learning and memory and astrocytes in old rats. Methods 50 Wistar rats were randomly divided into the sham group and model group with 25 animals in each group. All animals were assessed with Morris water maze to test the changes of abilities of learning and memory. The expressions of glial fibrillary acidic protein (GFAP) in the frontal lobe and hippocampus were observed immunohistochemically. Results Compared with the sham group, the scores of Morris water maze decreased in the model group, while the astrocytes marked by GFAP proliferated and enlarged significantly. Conclusion Proliferation and morphological changes of astrocytes are involved in pathological mechanism of chronic cerebral hypoperfusion, which might be associated with the decrease of ability of learning and memory.

Key words: cerebral hypoperfusion, learning and memory, glial fibrillary acidic protein (GFAP), rat