《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (08): 703-704.

• 专题 • 上一篇    下一篇

神经妥乐平对大鼠坐骨神经损伤后脊髓运动神经元的保护作用

齐巍1; 程爱国2; 刘昊2; 刘佳3   

  1. 1.华北煤炭医学院2006级版税学研究生,河北唐山市 063000;2.华北煤炭医学院附属医院骨外科,河北唐山市 063000;3.北京三博脑科医院护理部,北京市 100093
  • 收稿日期:2008-06-16 出版日期:2008-08-01 发布日期:2008-08-01

Possible Protective Effect and Mechanism of Neurotropin on Spinal Cord Motoneuron after Sciatic Nerve Injury in Rats

QI Wei,CHENG Ai-guo, LIUHao, et al   

  1. North China Coal Medical University, Tangshan 063000, Hebei, China
  • Received:2008-06-16 Published:2008-08-01 Online:2008-08-01

PDF (PC)

被引次数

8

摘要： 目的探讨大鼠坐骨神经损伤后早期应用神经妥乐平对相应脊髓运动神经元的保护作用。方法将108只雄性SD大鼠随机分为A组、B组和C组,每组36只,所有大鼠均行双侧坐骨神经切断后立即行神经外膜端-端吻合术。A组为对照组;B组术后予神经妥乐平治疗;C组术后予生理盐水治疗。按术后1d、4d、9d、14d、21d和28d6个时间点取L4～L6脊髓作Bcl-2、Bax免疫组化检测,以及TUNEL检测。结果B组术后9d、14d、21d的Bcl-2/Bax值较A、C组增高;B组与A、C组相比,9d的TUNEL阳性细胞数量有显著性差异(P<0.05),14d的TUNEL阳性细胞达高峰,其中B组的TUNEL阳性细胞数量少于A组和C组(P<0.05～0.01)。结论大鼠坐骨神经切断外膜端-端吻合术后,神经妥乐平可以一定程度地保护脊髓前角运动神经元避免其过度凋亡。

关键词: 坐骨神经损伤, 神经妥乐平, 神经元保护, 神经元凋亡, Bcl-2, Bax

Abstract: Objective To investigate the possible protective effect of neurotropin on the corresponding spinal cord motoneuron after sciatic nerve injury early in rats.Methods 108 male Sprague Dawley rats were divided randomly into Groups A,B and C with 36 animals in each group,and all animals were cut both side of sciatic nerve,instantly make the operation of end-to-end anastomose of epineurium for abscised sciatic nerve.Group A was control group.Group B was treated with neurotropin and Group C was treated with normal saline after operation.The rats were sacrificed on 1st d,4th d,9th d,14th d,21st d and 28th d after operation.The corresponding spinal cord segments(L4～L6)were gotten to make Bcl-2 and Bax immunohistochemical staining and TUNEL staining.Results The values of Bcl-2/Bax on 9th d,14th d and 21st d after operation of Group B were different from that of Group A and Group C.The number of positive cells stained by TUNEL staining on 9th d was significantly different among Group A,Group B and Group C(v<0.05).The expression of positive TUNEL staining cells reached a peak on 14th d after operation,the number of Group B was less than that of Group A and Group C.Conclusion After instantly make the operation of end-to-end anastomose of epineurium for abscised sciatic nerve,neurotropin can protect the spinal cord motoneurons to avoid apoptosis excessively in some extent.

Key words: sciatic nerve injury, neurotropin, neuron protection, neuron apoptosis, Bcl-2, Bax