《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (08): 727-728.

• 基础研究 • 上一篇    下一篇

乳化异氟醚预处理对缺血再灌注大鼠心肌的保护作用

张雪松; 曾繁荣; 左会明   

  1. 佳木斯大学附属第一医院麻醉科,黑龙江佳木斯市 154002
  • 收稿日期:2008-03-05 出版日期:2008-08-01 发布日期:2008-08-01

Protective Effect of Emulsified Isoflurane Preconditioned Intravenously on Myocardial Ischemia/Reperfusion Injury in Rats

ZHANG Xue-song, ZENG Fan-rong, ZUO Hui-ming   

  1. The Anesthesiology Department of the First Hospital Affiliated to Jiamusi University, Jiamusi 154002, Heilongjiang, China
  • Received:2008-03-05 Published:2008-08-01 Online:2008-08-01

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摘要： 目的观察乳化异氟醚预处理对缺血再灌注大鼠心肌的保护作用。方法随机将40只大鼠分为假手术组、生理盐水组、脂肪乳组和乳化异氟醚组,每组10只,建立大鼠心脏缺血再灌注模型。假手术组不结扎冠状动脉,其他3组分别于缺血再灌注前30min,静脉注入0.9%生理盐水、30%脂肪乳和6.9%的乳化异氟醚达30min(3.5ml/kg/h)。缺血30min再灌注3h后,检测心肌组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,HE染色观察心肌病理学变化,电镜观察心肌细胞的超微结构改变。结果缺血再灌注后,生理盐水组及脂肪乳组大鼠心肌MDA含量升高,SOD活性降低,与乳化异氟醚组相比差异有显著性意义(P<0.05)。结论乳化异氟醚预处理对缺血再灌注大鼠心肌有保护作用。

关键词: 异氟醚, 乳化, 缺血再灌注, 心肌, 大鼠

Abstract: Objective To investigate the protective effect of the emulsified isoflurane preconditioned intravenously on the myocardial ischemia/reperfusion injury in rats.Methods The 40 rats were randomly divided into the pseudo-operation group,saline group,lipid emulsion group and emulsified isoflurane group with 10 animals in each group.The animal model of myocardial ischemia reperfusion injury was established,the pseudo-operation group was not occluded coronary artery,the other three groups were received intravenous 0.9% saline,30% lipid emulsion,or infusions(3.5 ml/kg/h for 30 min)of emulsified isoflurane(6.9%)before a 30-min coronary occlusion and 3 h of reperfusion.The content of myocardial malondialdehyde(MDA)and the activity of superoxide dismutase(SOD)were determined after treatment.The myocardial samples were studied by HE staining to observe hispathological changes.The myocardial ultrastuctures were observed and pictures were taken by electron microscope.Results The myocardial MDA content in the emulsified isoflurane group was significantly lower than that in the saline group and lipid emulsion group,while the activity of SOD in the emulsified isoflurane group was significantly higher than that in the saline group and lipid emulsion group(P<0.05).Conclusion The emulsified isoflurane preconditioned intravenously has protective effect on myocardial ischemia/reperfusion injury in rats.

Key words: isoflurane, emulsification, ischemia/reperfusion, myocardial, rat