《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (08): 729-730.

• 基础研究 • 上一篇    下一篇

促红细胞生成素对大鼠脑缺血再灌注损伤后炎症反应的影响

陈勇军1; 汤永红2; 龙慧2   

  1. 1.南华大学附属南华医院神经内科,湖南衡阳市 421002;2.南华大学附属第二医院神经内科,湖南衡阳市 421001
  • 收稿日期:2008-02-21 出版日期:2008-08-01 发布日期:2008-08-01

Effect of Erythropoietin on Inflammation of Cerebral Ischemia-reperfusion Injury in Rats

CHEN Yong-jun, TANG Yong-hong,LONG Hui   

  1. The Department of Neurology, Nanhua Hospital Affiliated to Nanhua University, Hengyang 421002, Hunan,China
  • Received:2008-02-21 Published:2008-08-01 Online:2008-08-01

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摘要： 目的探讨促红细胞生成素(EPO)对大鼠局灶性脑缺血再灌注后炎症损伤的保护作用。方法54只SD大鼠随机分为假手术组、生理盐水对照组、EPO治疗组,采用线栓法制备大鼠大脑中动脉阻塞2h模型,再灌注6h、24h、48h。放免法检测各时间点白细胞介素1β(IL-1β)含量,比色法观察髓过氧化物酶(MPO)活性,同时观察24h时间点神经功能缺损评分。结果EPO组大鼠各时间点脑组织中的IL-1β含量和MPO活性降低(P<0.05),神经功能缺损评分明显降低(P<0.01)。结论EPO可能通过减少炎症因子IL-1β释放和抑制MPO活性,减轻炎症反应,对脑缺血再灌注损伤发挥保护作用。

关键词: 促红细胞生成素, 脑缺血再灌注, 炎症, 白细胞介素1β, 髓过氧化物酶

Abstract: Objective To explore the protective effect of erythropoietin(EPO)on inflammatory injury induced by focal cerebral ischemia/reperfusion in rats.Methods 54 male Sprague-Dawley rats were randomly divided into 3 groups:sham operation group,normal saline control group and EPO group.The focal cerebral ischemia-reperfusion injury model was made by suture-occluded method.The content of interleukin-1 β(IL-1β)and the activity of myeloperoxidease(MPO)were measured by radioimmunoassay and chromatoptometry at 6 h,24 h and 48 h reperfusion following ischemia 2 h.The scores of behavior obstacle in rats were assessed at 24 h.Results Compared with the normal saline control group,EPO group got better score of behavior obstacle(all P<0.05).The content of IL-1β and the activity of MPO in brain in the EPO group were significantly lower than those in the normal saline control group(all P<0.01).Compared with two reperfusion groups,the sham operation group had no obvious abnormal change in each measurement item.Conclusion EPO can reduce the content of IL-1β and inhibit the infiltration of leukoeyte,which can provide protective effect on cerebral ischemic-repefusion injury in rats.

Key words: erythropoietin, cerebral ischemia-repefusion, inflammatory, interleukin-1β, myeloperoxidase