《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (09): 801-803.

• 专题 •    下一篇

脑缺血预适应大鼠脑内p38丝裂原激活蛋白激酶磷酸化水平和蛋白表达量的测定

赵兰峰; 郭社卫; 安仰原; 刘明刚   

  1. 首都医科大学基础医学院神经生物学系,疼痛生物医学研究所 北京市 100069
  • 收稿日期:2008-07-04 出版日期:2008-09-01 发布日期:2008-09-01

Phosphorylation and Protein Expression of p38 Mitogen Activated Protein Kinases in Cerebral Ischemic Preconditioning Rat

ZHAO Lan-feng, GUO She-wei, AN Yang-yuan, et al   

  1. Institute for Biomedical Sciences of Pain, Department of Neurobiology, Capital Medical University School of Basic Medical Sciences, Beijing 100069, China
  • Received:2008-07-04 Published:2008-09-01 Online:2008-09-01

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被引次数

3

摘要： 目的初步探讨p38丝裂原激活蛋白激酶(p38 MAPK)在大鼠脑缺血预适应(IPC)形成中的作用。方法选用成年雄性Sprague-Dawley大鼠复制整体脑缺血预适应模型。将大鼠随机分为空白对照(control)、假手术(SI)、缺血预适应或缺血耐受(IT)、外周伤害对照(PNC)、外周伤害耐受(PNT)5组。应用SDS-PAGE、Western blot等实验方法,借助Gel Doc和Versa Doc凝胶成像系统,半定量检测脑躯体感觉皮层、海马组织内p38 MAPK的磷酸化水平和蛋白表达量。结果经脑IPC后,大鼠躯体感觉皮层和海马组织内p38 MAPK磷酸化水平和蛋白表达量与各自对照组相比均未见显著性差异(P>0.05,n=6)。结论大鼠大脑躯体感觉皮层和海马组织内p38 MAPK可能未以磷酸化水平和蛋白表达量改变的形式参与脑缺血预适应的形成。

关键词: 脑缺血预适应, p38丝裂原激活蛋白激酶(p38 MAPK), 磷酸化, 蛋白表达, 大鼠

Abstract: Objective To explore initially the role of p38 mitogen activated protein kinases(p38 MAPK) in cerebral ischemic preconditioning.Methods Healthy adult SD rats were randomly divided into 5 groups: normal control group,sham-operated group,ischemia preconditioning or ischemia tolerance group,peripheral noxious control group,peripheral noxious tolerance group.SDS-PAGE,Western blot and Gel Doc imagine systems were applied to determine the p38 MAPK phosphorylation and protein expression in somatosensory cortex and hippocampus of rat.Results No significant changes of p38 MAPK in phosphorylation level and protein expression were found both in somatosensory cortex and hippocampus after ischemia preconditioning(P>0.05,n=6).Conclusion The development of cerebral ischemia preconditioning of rat might be not involved the phosphorylation and protein expression of p38 MAPK.

Key words: cerebral ischemia preconditioning, p38 mitogen activated protein kinases(p38 MAPK), phosphorylation, protein expression, rats