《中国康复理论与实践》

《中国康复理论与实践》 ›› 2008, Vol. 14 ›› Issue (09): 804-805.

• 专题 • 上一篇    下一篇

吡咯喹啉醌对培养的海马神经元衰老的影响

童希文1a; 韩艳酶1a; 邓永辉1a; 沈慧1a; 熊顺华1b   

  1. 1.郧阳医学院a.药护学院;b.生理学教研室湖北十堰市 442000
  • 收稿日期:2008-03-24 出版日期:2008-09-01 发布日期:2008-09-01

Effect of Pyrroloquinoline Quinone on Aging of Rat Hippocampal Neurons in Vitro

TONG Xi-wen, HAN Yan-mei, DENG Yong-hui, et al   

  1. Department of Physiology, Yunyang Medical College, Shiyan 442000, Hubei, China
  • Received:2008-03-24 Published:2008-09-01 Online:2008-09-01

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摘要： 目的观察吡咯喹啉醌(PQQ)对D-半乳糖(D-gal)致培养大鼠海马神经细胞衰老损伤的影响。方法体外原代培养新生大鼠海马神经元,用大剂量D-gal诱致海马神经元老化损伤,用PQQ做保护研究,镜下观察神经元的形态变化,测海马神经细胞自由基的水平,流式细胞仪测细胞的凋亡率,免疫组化测Bax蛋白的表达。结果体外培养8 d的海马神经细胞用D-gal处理后,细胞自由基水平升高,细胞的死亡率增加,Bax表达增强;预先给予PQQ处理后,细胞内自由基水平以及Bax的表达下降,细胞死亡率下降。结论PQQ能廷缓D-gal引起的培养大鼠海马神经细胞的衰老。

关键词: 吡咯喹啉醌(PQQ), 海马, 神经元, 衰老, D-半乳糖(D-gal), 大鼠

Abstract: Objective To investigate the effect of pyrroloquinoline quinone(PQQ) on the aging of rat hippocampal neurons induced by D-galactose(D-gal).Methods Hippocampal neurons were cultured in vitro.The aging of the hippocampal neurons was induced by high dose D-gal,PQQ protection were used 30 min before D-gal.The metamorphosis of hippocampal neurons was observed under the microscope.The contents of free radical was measured.The incidence of apoptosis of hippocampus cells was tested with the flow cytometry.The expression of Bax was detected with immunohistochemical staining.Results After the cells cultured in vitro exposed to D-gal,the content of free radical and the expression of Bax of the hippocampal neurons increased.After pretreatment of the cultured neurons with PQQ,the contents of free radical and the expression of Bax decreased,the survival of hippocampal neurons increased.Conclusion PQQ may slow the aging progress of hippocamal neurons induced by D-gal.

Key words: pyrroloquinoline quinone(PQQ), Hippocampus, neurons, Aging, D-galactose(D-gal), rats