《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (01): 41-43.

• 基础研究 • 上一篇    下一篇

益气活血复方对兔颈总动脉球囊损伤后基质金属蛋白酶-2 mRNA表达的影响

刘会武1a;张艳1b;孙江波1a   

  1. 1.辽宁中医药大学，a.研究生学院;b.附属医院，辽宁沈阳市 110032
  • 收稿日期:2008-07-17 出版日期:2009-01-01 发布日期:2009-01-01
  • 通讯作者: 张艳

Effect of YQHXFF on Expression of Matrix Metalloproteinase-2 after Percutaneous Transluminal Coronary Angioplasty in Rabbits with Hyperlipidemia

LIU Hui-wu, ZHANG Yan,SUN Jiang-bo   

  1. School of Graduate, Department of Internal Medicine, Hospital Affiliated to Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China
  • Received:2008-07-17 Published:2009-01-01 Online:2009-01-01

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摘要： 目的 观察益气活血复方对兔颈动脉损伤后血管内膜增生及基质金属蛋白酶-2（MMP-2） mRNA表达的影响。方法 将雄性新西兰白兔28只，随机分为3组，空白组、模型组和益气活血复方组。第8周末处死动物，用RT-PCR法检测损伤处颈总动脉组织中MMP-2 mRNA的表达，HE染色观察颈动脉组织的病理形态学改变。结果 与模型组相比，益气活血复方可明显抑制损伤后的颈总动脉内膜的增殖反应，下调损伤处组织中MMP-2 mRNA表达的水平(P<0.05)。结论 益气活血复方具有抑制动脉内膜增殖、下调MMP-2 mRNA表达的作用。

关键词: 益气活血复方, 经皮腔内冠状动脉成形术(PTCA), 基质金属蛋白酶-2 （MMP-2）

Abstract: Objective To observe the effects of YQHXFF on the expression of matrix metalloproteinase-2 （MMP-2） in the rabbits with hyperlipidemia after percutaneous transluminal coronary angioplasty (PTCA). Methods 28 male New Zealand white rabbits were randomly divided into the control group, the model group and the YQHXFF intervention group. The models of restenosis were established with injuring carotid arteries in cholesterolfied rabbits. After 8 weeks, MMP-2 were measured with reverse transcription polymerize chain reaction (RT-PCR) and pathologic alter were observed with HE staining. Results The expression of MMP-2 mRNA in injured artery were much higher in the model group. Compared with those in the model group, the expression of MMP-2 in the YQHXFF intervention group markedly decreased and intimal hyperplasia markedly lighten. Conclusion YQHXFF can inhibit the expression of MMP-2 and lighten the intimal hyperplasia after PTCA.

Key words: YQHXFF, percutaneous transluminal coronary angioplasty (PTCA), matrix metalloproteinase-2 （MMP-2）