《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (01): 44-47.

• 基础研究 • 上一篇    下一篇

自体骨髓间充质干细胞移植与纤维蛋白胶复合骨形态发生蛋白体内成骨的比较研究

常玉立1,2;孙天胜1;刘智1;叶超群1;余兆仲1;张志成1;任大江1   

  1. 1.北京军区总医院全军创伤骨科研究所，北京市 100700;2.总后勤部管理保障局第一门诊部，北京市 100842
  • 收稿日期:2008-08-07 修回日期:2008-09-18 出版日期:2009-01-01 发布日期:2009-01-01
  • 通讯作者: 孙天胜

Comparison of Osteogenic Potential in vivo between Autologous Bone Marrow Mesenchymal Stem Cell Implanting and Fibrin Sealant Combined with Recombined Human Bone Morphogenetic Protein-2 Grafting

CHANG Yu-li, SUN Tian-sheng, LIU Zhi, et al   

  1. PLA Institute of Orthopaedics and Traumatology, Beijing Army General Hospital, Beijing 100700, China
  • Received:2008-08-07 Revised:2008-09-18 Published:2009-01-01 Online:2009-01-01

PDF (PC)

被引次数

18

摘要： 目的 比较纤维蛋白胶(FS)复合骨形态发生蛋白-2(BMP-2)与各种自体骨髓间充质干细胞(BMSCs)移植骨折后体内成骨的效果。方法 20只日本大耳白兔行桡骨中段骨缺损造模。取第2代BMSCs进行成骨诱导。分别将BMSCs联合成骨诱导后的BMSCs（A组）、成骨诱导的BMSCs(B组)、BMSCs(C组)、生理盐水(E组)经皮注入兔桡骨中段骨缺损处，FS复合BMP-2于造模手术中植入(D组)。通过组织学、放射学、生物力学方法在不同时相比较骨缺损区修复情况。结果 A组在12周内骨缺损区新生骨的数量和质量与D组无显著性差异（P>0.05），但两组均优于B组（P<0.01）和C组（P<0.01），E组骨缺损主要由纤维结缔组织填充。结论 BMSCs联合成骨诱导后的BMSCs、FS复合BMP-2均具有理想的体内成骨能力；BMSCs与成骨诱导后的BMSCs联合移植可能存在协同效应，具有更强的成骨能力，可作为种子细胞应用于骨组织工程。

关键词: 纤维蛋白胶, 骨形态发生蛋白-2(BMP-2), 骨髓间充质干细胞, 细胞移植, 骨缺损

Abstract: Objective To investigate the osteogenic potential for size-critical bone defect of fibrin sealant combined with recombined human bone morphogenetic protein-2 (rhBMP-2) grafting and varied autologous bone marrow mesenchymal stem cells (BMSCs) implanting in vivo. Methods BMSCs were cultured and induced with osteogenic supplement (OS) medium. BMSCs with and without OS induction were collected and percutaneously autologous injected respectively into the 15 mm bone defect of experimental rabbit model. The grafts were BMSCs, osteo-induced BMSCs, BMSCs and osteo-induced BMSCs, BMP combined with fibrin sealant, 0.9% NaCl solution. Osteogenesis at the defect area was assessed with regular radiography, histology and biomechanics. Results The FS/BMP group and the BMSCs+osteo-induced BMSCs group achieved complete bone healing with medullary cavity united, with the most new bone formation and the maximal load among those groups. Conclusion The osteogenic potential of both osteo-induced BMSCs combined with BMSCs and FS/BMP are similar, which are superior to that of BMSCs or osteo-induced BMSCs along.

Key words: fibrin sealant, bone morphogenetic protein-2(BMP-2), bone marrow mesenchymal stem cells (BMSCs), cells implanting, bone defection