《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (02): 123-125.

• 基础研究 • 上一篇    下一篇

淫羊藿黄酮对阿尔茨海默病模型神经炎性反应的影响

林丽莉1;宋秋洁1,2;叶翠飞1;张丽1;李林1   

  1. 1. 首都医科大学宣武医院药物研究室，神经变性病教育部重点实验室，北京市 100053;2. 国家食品药品监督管理局药品评价中心，北京市 100045
  • 收稿日期:2008-10-16 出版日期:2009-02-01 发布日期:2009-02-01
  • 通讯作者: 李林

Effect of Epimedium Flavanoids on Neuro-inflammatory Reaction in Alzheimer Disease Model Mice Induced by Lateral Ventricle Injection of β-amyloid

LIN Li-li, SONG Qiu-jie, YE Cui-fei, et al   

  1. The Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China
  • Received:2008-10-16 Published:2009-02-01 Online:2009-02-01

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摘要： 目的 观察淫羊藿黄酮（EF）对阿尔茨海默病(AD)小鼠模型神经炎性反应的影响。方法 2月龄ICR雄性小鼠侧脑室注射β淀粉样肽1-40(Aβ)，建立AD小鼠模型。应用Morris水迷宫法和避暗试验检测小鼠的学习记忆功能，放射免疫法测定脑内白细胞介素1β（IL-1β）和肿瘤坏死因子α（TNF-α）含量，免疫组织化学法测定脑内神经胶质元纤维酸性蛋白（GFAP）阳性细胞（星形胶质细胞）的表达。结果 与对照组比较，模型组小鼠学习记忆能力降低（P<0.05，P<0.01），脑内IL-1β和TNF-α含量增加(P<0.01)，星形胶质细胞增生(P<0.05)。与模型组比较，EF给药组（100 mg/kg、300 mg/kg）小鼠的学习记忆能力明显改善（P<0.05，P<0.01），IL-1β和TNF-α含量及GFAP阳性细胞表达数均降低（P<0.05，P<0.01）。结论 EF可降低Aβ诱导的小鼠脑内炎性反应。

关键词: 淫羊藿黄酮, 阿尔茨海默病, β淀粉样肽, 炎症反应

Abstract: Objective To investigate the effect of Epimedium flavanoids (EF) on neuro-inflammatory reaction in Alzheimer disease (AD) mice model.Methods β-amyloid1-40 (Aβ1-40) was injected to the right lateral ventricle of 2-month-old male ICR mice to induce AD model. Morris water maze and step-through tests were used to measure the learning and memory ability of mice. The concentrations of interleukin-1β (IL-1β) and tumor necrosis factor α (TNF-α) in the hippocampus were determined by radioimmunoassay. The expression of glial fibrillary acidic protein (GFAP) positive cells (astrocytes) was detected by immunochemistry staining.Results Compared with the sham-operation group, the learning and memory ability decreased (P<0.05, P<0.01), the concentrations of IL-1β and TNF-α in the hippocampus increased (P<0.01) and the expression of astrocytes in the hippocampus elevated (P<0.05) in Aβ1-40 injection model mice. Intragastric administration of EF (100 and 300 mg/kg) for 3 weeks significantly improved the learning and memory ability (P<0.05, P<0.01), decreased IL-1β and TNF-α concentrations in the hippocampus (P<0.05, P<0.01) and inhibited the expression of astrocytes in the hippocampus (P<0.05), compared with Aβ1-40 injection model mice.Conclusion EF can decrease the inflammatory reaction in the brain of mice induced by Aβ.

Key words: Epimedium flavanoids, Alzheimer disease, β-amyloid, inflammation