《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (02): 126-128.

• 基础研究 • 上一篇    下一篇

淫羊藿黄酮对APP695基因转染神经细胞β-淀粉样肽及β-分泌酶的影响

杨潘1;宋秋洁1,2;王月华3;李雅莉1;杜冠华3;李林1   

  1. 1. 首都医科大学宣武医院药物研究室，神经变性病教育部重点实验室，北京市100053;2. 国家食品药品监督管理局药品评价中心，北京市 100045;3. 中国医学科学院药物研究所，北京市 100050
  • 收稿日期:2008-10-16 出版日期:2009-02-01 发布日期:2009-02-01
  • 通讯作者: 李林

Effects of Epimedium Flavanoids on β-amyloid in APP695 cDNA-transfected Nerve Cells and on β-secretase Activity in Vitro

YANG Pan, SONG Qiu-jie, WANG Yue-hua, et al   

  1. The Department of Pharmacology, Xuanwu Hospital of Capital Medical University, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing 100053, China
  • Received:2008-10-16 Published:2009-02-01 Online:2009-02-01

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摘要： 目的 观察淫羊藿黄酮（EF）对APP695基因转染SH-SY5Y细胞中β-淀粉样肽（Aβ）水平的影响。方法 利用免疫沉淀+Western印迹法、放射免疫法分别检测细胞培养上清液和细胞裂解液中的Aβ水平。通过测定β-分泌酶（BACE1）切割荧光底物所得的荧光值检测BACE1活性。结果 EF 2.5～25 g/ml与APP695基因转染SH-SY5Y细胞模型共孵育24 h能明显降低模型细胞培养液中的Aβ含量（P<0.05，P<0.01）。体外试验中EF 50 g/ml对BACE1活性的抑制率为95.0%。结论 EF能降低APP695基因转染细胞生成和分泌Aβ。

关键词: 淫羊藿黄酮, β-淀粉样肽, β-分泌酶, 阿尔茨海默病

Abstract: Objective To investigate the effects of Epimedium flavanoids (EF) on β-amyloid (Aβ) in APP695 cDNA-transfected human neuroblastoma cell line SH-SY5Y cells.Methods The immunoprecipitation plus Western blotting and radioimmunoassay were used to detect Aβ concentration in the supernatant of cell culture and cell disruption fluid. The activity of β-secretase (BACE1) was measured by detecting the fluorescence value of substrate cut by BACE1.Results Incubation of EF 2.5～25 g/ml with APP695 cDNA-transfected SH-SY5Y cells for 24 h significantly decreased Aβ content in the supernatant of cell culture (P<0.05, P<0.01). EF 50 g/ml inhibited the activity of BACE1 by 95.0% in vitro.Conclusion EF can decrease Aβ production and secretion of APP695 cDNA-transfected nerve cells.

Key words: Epimedium flavanoids, β-amyloid, β-secretase, Alzheimer disease