《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (02): 129-131.

• 基础研究 • 上一篇    下一篇

大鼠脑外伤诱导反应性星形胶质细胞表达水通道蛋白1和水通道蛋白9

张琳1;袁芳2   

  1. 1. 首都医科大学附属北京天坛医院科研处，北京市 100050;2. 北京市神经外科研究所病理生理室，北京市 100050
  • 收稿日期:2008-10-31 出版日期:2009-02-01 发布日期:2009-02-01
  • 通讯作者: 袁芳

Aquaporin-1 and Aquaporin-9 Expressed by Reactive Astrocytes after Traumatic Brain Injury in Rat

ZHANG Lin, YUAN Fang   

  1. The Department of Scientific Research Management, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China
  • Received:2008-10-31 Published:2009-02-01 Online:2009-02-01

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摘要： 目的 研究水通道蛋白1（AQP1）和水通道蛋白9（AQP9）在大鼠自由落体脑损伤后的表达变化以及与脑水肿的关系。方法 复制大鼠自由落体脑损伤模型，在外伤后1 h、6 h、24 h、48 h和168 h用干重-湿重法测定损伤侧脑组织水含量，利用免疫组化染色和Western印迹法检测AQP1和AQP9表达的变化。结果 脑外伤后损伤侧脑组织水含量增加，24 h达高峰，168 h接近恢复。脑外伤后6 h损伤周围出现AQP1和AQP9免疫染色阳性的星形胶质细胞，外伤后24 h免疫染色阳性细胞数最多，且损伤周围处血管内皮细胞AQP1表达异常增加。结论 脑外伤诱导AQP1和AQP9的表达，共同参与脑水肿的发展过程。

关键词: 脑损伤, 脑水肿, 水通道蛋白1, 水通道蛋白9

Abstract: Objective To investigate the expression changes of aquaporin-1 (AQP1) and aquaporin-9 (AQP9) in rats after traumatic brain injury, and explore the roles of AQP1 and AQP9 in the development of brain edema.Methods In an impact-acceleration head injury model of rat, brain water content was measured by wet-dry weight method at 1 h, 6 h, 24 h, 48 h and 168 h after brain trauma. The expressions of AQP1 and AQP9 in brains were investigated by immunohistochemistry and Western blot.Results Brain water content increased in the injuried brain tissues after 1 h post-injury, reached peak at 24 h and returned at 168 h. Both expressions of AQP1 and AQP9 were induced in reactive astrocytes adjacent to injury sit at 6 h and 24 h after brain trauma. Staining of AQP1 in the endothelial cells was not present in normal rat brains but appeared strong after brain trauma.Conclusion A remarkable induction expression of AQP1 or AQP9 after brain trauma may participate in the development of brain edema.

Key words: traumatic brain injury, brain edema, aquaporin-1, aquaporin-9