《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (03): 203-205.

• 专题 • 上一篇    下一篇

天麻提取物在锂-匹罗卡品癫痫大鼠中的神经保护作用

王本国1;杨楠1;廖卫平2;苏涛2   

  1. 1.广州中医药大学中山附禹医院神经内科，广东中山市528400;2.广州医学院神经科学研究所，广东广州市 510260
  • 收稿日期:2008-10-27 出版日期:2009-03-01 发布日期:2009-03-01

Neuroprotection of Extraction of Gastrodia Elata for Status Epilepticus Induced by Li-Pilocarpin

WANG Ben-guo, YANG Nan, LIAO Wei-ping, et al   

  1. Department of Neurology, Zhongshan affiliated Hospital of Guangzhou University of Traditional Chinese Medicine, Zhongshan 528400, Guangdong, China
  • Received:2008-10-27 Published:2009-03-01 Online:2009-03-01

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摘要： 目的 探讨天麻提取物在锂-匹罗卡品癫痫大鼠模型中的神经保护作用。方法 SD大鼠144只，随机分成盐水对照组、小剂量天麻组、大剂量天麻组及拉莫三嗪组，分别予生理盐水5 ml/kg、天麻醇提取液5 ml/kg、天麻醇提取液10 ml/kg及拉莫三嗪20 mg/kg灌胃，每日2次，共14 d。治疗3 d后制作锂-匹罗卡品癫痫持续状态模型。比较癫痫持续状态时间、海马及齿状回神经元丢失、癫痫自发发作及齿状回内分子层苔藓纤维出芽。结果 与对照组及小剂量天麻组比较，大剂量天麻及拉莫三嗪能缩短癫痫持续状态时间，减少海马CA1、CA3区及齿状回门区神经元丢失，但所有治疗均不能阻止齿状回苔藓纤维出芽及癫痫的自发发作。结论 20 ml/kg天麻醇提取物在锂-匹罗卡品模型中能缩短癫痫持续状态发作的时间，减轻海马CA1、CA3及齿状回门区神经元丢失，同40 mg/kg拉莫三嗪一样可发挥神经保护作用，但不能阻断癫痫的发生发展过程。

关键词: 癫痫持续状态, 天麻, 神经保护, 拉莫三嗪

Abstract: Objective To investigate the neuroprotective effect of extraction of gastrodia elata on status epilepticus induced by Li-pilocarpine in rats. Methods 144 SD rats were randomly divided into 4 groups: saline control (5 ml/kg twice daily), small dose gastrodia group (5 ml/kg twice daily), large dose gastrodia group (10 ml/kg twice daily) and lamotrigine group (20 mg/kg twice daily). The status epilepticus was induced 3 d after treatment. The duration of status epilepticus, neuron loss, the rate of spontaneous seizure and the score of mossy fiber sprouting were analyzed. Results Large dose gastrodia or lamotrigine administration can shorten the duration of status epilepticus and decrease the neuron loss compared with the small dose gastrodia and control administration. But all treatments cannot prevent mossy fiber sprouting and spontaneous seizures. Conclusion 20 ml/kg gastrodia or 40 mg/kg lamotrigine administration can shorten the duration of status epilepticus induced by Li-pilocarpine, and decrease the neuron loss of CA1, CA3 of hippocampus and hilus of dentate gyrus, but cannot prevent epileptogenesis after brain insult.

Key words: status epilepticus, gastrodia elata, neuroprotection, lamotrigine