《中国康复理论与实践》

《中国康复理论与实践》 ›› 2009, Vol. 15 ›› Issue (03): 220-222.

• 基础研究 • 上一篇    下一篇

探索环境对局灶性脑梗死大鼠行为学恢复及梗死灶周围皮层神经颗粒素表达的影响

槐雅萍1;贾新凤2;贾子善1   

  1. 1.河北省人民医院康复医学科，河北石家庄市 050051;2.天津大港油田总医院，天津市 300280
  • 收稿日期:2008-09-27 修回日期:2008-12-09 出版日期:2009-03-01 发布日期:2009-03-01
  • 通讯作者: 贾子善

Effect of Exploratory Environment on Behavioral Recovery and Expression of Neurogranin in Rats after Unilateral Local Cerebral Infarction

HUAI Ya-ping, JIA Xin-feng, JIA Zi-shan   

  1. Department of Rehabilitation Medicine, Hebei Provincial People's Hospital, Shijiazhuang 050051，Hebei, China
  • Received:2008-09-27 Revised:2008-12-09 Published:2009-03-01 Online:2009-03-01

PDF (PC)

被引次数

9

摘要： 目的 观察探索环境对局灶性脑梗死大鼠行为学恢复及神经颗粒素（Ng）表达的影响。方法 健康雄性SD大鼠70只，其中60只电凝法造成右侧大脑中动脉阻断（MCAO）模型后，随机分为探索组（n=30，置于迷宫笼喂养）和手术对照组（n=30，置于普通宠喂养），另10只为假手术组。各实验组随机取10只，分别于术后1 d、7 d、14 d、28 d时进行Berderson神经功能评分、肌力试验及平衡木试验。探索组和手术对照组分别于术后1 d、7 d、14 d、28 d处死5只大鼠，假手术组分别于7 d、28 d时处死5只大鼠，进行Ng免疫组化染色。结果 探索组行为学评分于14～28 d优于手术对照组（P<0.05）。Ng阳性神经元数在7 d、14 d、28 d明显多于手术对照组（P<0.01）。结论 探索环境可促进大鼠行为学功能的恢复并促进梗死灶周围皮层Ng表达。

关键词: 探索环境, 脑梗死, 行为, 神经颗粒素(Ng), 大鼠

Abstract: Objective To study the effect of exploratory environment on behavioral recovery and the expression of neurogranin (Ng) in rats after unilateral local cerebral infarction. Methods 70 male Sprague-Dawley rats were adopted. After making the model of MCAO with electric coagulation, 60 rats were randomly divided into exploratory group (n=30, living in maze cages) and control group (n=30, living in large standard cages). Other 10 rats as the sham group lived in standard cage. 10 rats were chosen randomly from the groups for Berderson test, manual muscle and balancing test on the 1st, 7th, 14th, 28th day after operation, and 5 rats were sacrificed separately in exploratory group and control group at the same time. 5 rats in sham group were randomly sacrificed separately on the 7th, 28th day after operation. The expressions of Ng in the peri-ischemic cortex were detected with immunohistochemistry stain. Results The scores of behavior in exploratory group improved compared with that in control group (P<0.05) from 14 d after operation. The number of Ng positive cells increased in exploratory group compared with that in control group (P<0.01) from 7 d after operation. Conclusion Exploratory environment can facilitate the functional recovery and the Ng expression in rats after unilateral local cerebral infarction.

Key words: exploratory environment, cerebral infarction, behavior, neurogranin (Ng), rats